The Chemical Constituents Of Elsholtzia Blanda And Studies On Separation And Purification Of Total Flavones From It

This thesis is composed of two parts. Part Ⅰ includes a research review of Elsholtzia plant, and studies on the chemical constituents of Elsholtzia blanda. In this part, 24 chemical compounds were isolated from E. blanda, among which 18 chemical compounds had been identified by multi-spectral techniques (1D and 2D NMR, ESI-MS). Part Ⅱ mainly introduces activities of flavonoids and a research about total flavones separation and purification of E. blanda.Part Ⅰ The Chemical Constituents of Elsholtzia blandaElsholtzia blanda named Silenghao, Dixiangru, Jigansan and so on, is a Chinese medicinal plant, which is distributed in Yunnan, Guizhou and Guangxi Province of China. It is a daily medicine of local Lahu in Xishuangbanna of Yunnan province. E. blanda, belonging to Elsholtzia genus of Labiatae, is the perennial herb with fragrance, whose leaves, flowers or whole grass are used as medicine. Its character is bitter, peppery, cool and having the effect on reducing fever, anti-inflammation, improving eyesight, diuresis. E. blanda cures mainly nyctalopia, dysentery, cold, angina, tonsillitis, acute gastroenteritis, acute pyelonephritis, hepatitis, presbyopia, etc. In order to utilize the abundant plant resource of E. blanda and find out the active constituents of E. blanda, 50% ethanol extracts of E. blanda was isolated and 24 compounds were obtained, among which 18 chemical compounds were characteristed by ESI-MS, 1D NMR, 2D NMR as apigenin-6,8-di-C-β-D-glucopyranoside(1), luteolin-7-O-β-D-glucopyranoside(2),luteolin-5-O-β-D-glucopyranoside(3),luteolin(4), 5-hydroxy-7,8-dimethoxyflavone(5), 5-hydroxy-6,7-dimethoxyflavone(6), 5-hydroxy-7-methoxyflavone(7),5,7-dihydroxy-6,8-dimethoxyflavone(8),5,8-dihydroxy-7-methoxyflavone(9), 4,4'-methylenebis[phenethyl alcohol](10), β-sitosterol(11), stigmasterol(12), β-sistosterol-3-O-β-D-glucopyranoside(13), ursolic acid(14), oleanolic acid(15), 2β,3β,23-trihydroxyolean-30-methoxycarbonyl-11,13(18) -diene-28-oic acid(16), 2β,3β,23-trihydroxyolean-30-methoxycarbonyl-12-ene-28-oic acid(17), 2β,3β,23-trihydroxyolean-12-ene-28,30-dioic acid(18). Among themcompound 16 was a new chemical compound, compound 1, 7, 8, 9, 10, 11, 17 and 18were isolated from Elsholtzia plant for the first time.Part II Studies on separation and purification of the total flavoncs fromElsholtzia blandaFlavonoids, including the basic structure of C6-C3-C6, are the plant secondary metabolites, and exist in the natural plant in the form of dissociate or glucoside. Not only are flavonoids quantity and sort various, but also their structure type are complicated. These characteristics determaine the activities of flavonoids on respiratory system disease, heart and brain blood-vascular disease, such as anti-inflammation, restraining fungus, anti-oxidant, anti-radiation, anti-tumor and strengthening immune ability, etc. In order to explore the abundant resources of E. blanda and look for the effective chemical component on cardiovascular disease, a research on the total flavones separation and purification of E. blanda had been carried through. Finally, a simple and feasible method was found and used for industrialized production.