Effect Of The Bu Shen Huo Xue Decoction On PCO Rats

Objective: The aim of this study was to investigate the effects of Bu Shen Huo Xue Decoction(BSHXT) in rats with polycystic ovary and to explore the possible mechanism of BSHXT in cells and molecules levels. Ovarian morphology, serum hormone, apoptosis of granulosa cells ,atresia of follicles and expression of Bcl-2 and Bax were observed with optic microscope, radio-immunity immunological histo chemistry, terminal deoxynucleotidy transferase-mediated dUTP nick end labeling(TUNEL), and immunohistochemical staining.Material and method: 24-day-old immature female SD rats (n=60) were separated into two groups at random. One group was PCOS animal model(n= 50), the other group was used as control(n=10). progesterone-synchronized immature rats were used as the animal model for Polycystic ovary (PCO) by injecting 1.5 IU hCG twince daily to 36 days old. And then separated 3 groups (11 rats per group) from the model group, treated with BSHXT for 30 days, separated 7 rats from the model group as the control group.After treatment, killed the rats. Blood specimens were centrifuged at 3000r /min for 20 minutes, the supernatant was collected for measuring E2 ,P ,T.Weighed the ovarian weight, measured the ovarian long diameter and short diameter, estimated ovarian volume and mean ovarian product (MOP).Counted the number of luteum and cystic follicles, measured the thickness of thecal cells and granulosa cells in general HE staining section.The apoptosis of granulosa cells was checked by TUNEL. Brown drops in nucleolus were regarded as the positive staining. Count the number of apoptosis of granulosa cells and atresia of follicles. Measure the diameter of atresic follicles.The expressions of Bcl-2 and Bax were checked by immunohistochemical staining. Light brown drops in cells were regarded as the positive staining. Five sights were selected in 400X sight at random, the score of positive cells/all cells ratio and positive cell's staining intensity was counted. Added up the two scores and got the positive integral.Statistics: Quantitative data were expressed as mean ± SE. Comparison between groups was undertaken using paired t-tests. Percentage data were undertaken using u-tests. P<0.05 was defined as statistically significant.