| OBJECTIVE To investigate the effects of various degrees of REM sleep deprivation (RSD) and recovery sleep (RS) on cognitive function and the expression of genes related to synaptic plasticity in rat hippocampus. Methods: The modified multiple platform method (MMPM) was used to establish sleep deprivation model. The cognitive function was tested by Y-type maze, the total error reaction number (EN) that each animal made within an experimental day and the total reaction time (TRT) were recorded. Sytâ…£, BDNF, TrkB mRNA levels were detected by real time PCR and their protein levels were detected by immunohistochemistry. Results: (1) EN increased significantly after RSD and RS. (2) TRT decreased at RSD1d and RSD3d, and increased significantly after RSD7d. (3) Sytâ…£ expression was significantly increased in RSD 1d, 3d groups and decreased with prolonging of RSD time (4)BDNF and TrkB expressions were significantly increased in RSD 1d, reached the peak 3 days after RSD, then decreased gradually with prolonging of RSD time. |
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