| Epilepsy is a very common neurological disease,which is characterized by transient and recurrent central nervous system(CNS)function abnormalities caused by the abnormal discharge of brain neurons.At present,there are about 50-70 million people suffering from different types of epilepsy in the world,of which about4.6 million new cases are added each year,and the number of new epilepsy patients in my country has reached 400,000 each year.There are many reasons for patients’ illness,such as stroke,brain injury,central nervous system infection and parasite infection.In addition,there are many patients whose causes cannot be identified.At present,there is a lack of effective treatments for epilepsy.Some patients with certain lesions can be treated by the surgical method of partial resection of brain tissue,but the resection surgery has a higher risk and is very likely to affect the normal function of the patient’s brain.Most patients with unknown lesions can only suppress or slow down the onset of epilepsy by taking anti-epileptic drugs.The above situation emphasizes the importance of in-depth research on the pathogenesis of epilepsy and the development of pathological processes through animal models.At present,the animal model closest to the mechanism of human temporal lobe epilepsy is the kainic acid temporal lobe epilepsy model.Many studies in the world use it to simulate the process of complex partial epileptic seizures in humans.At the same time,many studies at home and abroad have revealed that BDNF,as a member of the neurotrophin family,can play an extremely important role in the regulation of the nervous system,and BDNF and its receptors play an important role in the hippocampus,a sensitive area of epileptic seizures.The expression level is the highest,so this study decided to use the BDNF-TrkB signaling pathway as an entry point to study the changes in synaptic plasticity of hippocampal neurons in epileptic rats.In this study,a rat model of kindling epilepsy was successfully established by the method of intracerebroventricular stereotactic injection of kainic acid.The reason why this method is used for research is that it has good repeatability and stable modeling.In preliminary observations,this study used Nissl staining and transmission electron microscopy to conduct histological observations on rat brain tissue slices.It was found that the injured area of epileptic rats was mainly concentrated in the CA3 area,and mainly manifested as CA3 pyramidal cells.In order to further explore the expression of BDNF-TrkB in the hippocampus of epileptic rats and its possible relationship with synaptic plasticity-related proteins MAP-2,SYP and PSD-95,this study used Western blot,immunohistochemical staining,and transmission electron microscopy have further studied the hippocampus of epileptic rats.It is found that the number of synapses in the model group is significantly less than that in the normal control group,and there is a certain connection between BDNF-TrkB and synaptic plasticity.In order to further study the possible relationship between the two,and on the basis of previous research,use the specific blocker of TrkB K252 a to block the BDNF-TrkB signaling pathway,and then the signaling pathway and synaptic plasticity related proteins were tested,and the results showed that the expression of synaptic plasticity-related proteins SYP,PSD-95 and MAP-2 all decreased,and the expression of MAP-2 decreased particularly significantly.In summary,this study believes that the BDNF-TrkB signaling pathway is involved in the regulation of synaptic plasticity in the hippocampus of epileptic rats by influencing the expression of prominent markers SYP,PSD-95 and MAP-2.May be used as a new target for the treatment of temporal lobe epilepsy. |
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