Expression Of E-cadherin And β-catenin In Colorectal Carcinoma And Clinical Significance

Objective: We performed E-cadherin andβ-catenin expression investigation with immunohistochemical method on colorectal cancer in order to elucidate whether the expression of E-cadherin andβ-catenin correlate with clinicopathological variables and prognosis.Materials and methods: The specimens of cancer tissues were taken from 139 consecutive patients with colorectal adenocarcinoma who had undergone resection from July to December in 1999 at liaoning Cancer Hospital. And the specimens of normal tissues were taken from the resected colorectal spicemens above 10cm far from cancer. Four micrometers thick sections prepared from formalin-fixed and paraffin-embedded tumor tissue or normal tissue on the slides were stained immunohistochemically with the primary antibodies as mouse monoclonal anti-human E-cadherin orβ-catenin (Fu Zhou Maxim Biotechnology, ready to use) and the SP kit(Fu Zhou Maxim Biotechnology) . Nomal mucosal epithelium served as an internal positive control and adjacent normal mucosal epithelium as an internal positive control . Negative control was obtained by replacing the primary antibody with PBS. Based of the previous immunhistochemical studies,the membranous staining of cells were positively stained with brown by the antibody. Calculating the percentage of positively membranous stained cells evaluated for each tissue section after counting 100 cells at more than 5 high-power(×400) fields. The Expression of E-cadherin andβ-catenin were classified as follows:If more than 90% of the tumor cells were positively membranous stained , the membranous expression was judjed to be normal; If less than 90% of the tumor cells...