On-line Coupling Of Capillary Electrophoresis And Chemiluminescence Detection Technique For Pharmaceutical Analysis

It is of great significance to study drugs and their metabolites. According to the drug characteristics about their biotransformation in organism, information about drug distribution and metabolism was acquired. With this information, the way in which drug metabolized was comprehended. It provided useful suggestions and evaluated those drug qualities through many aspects studies, such as production, experiment and clinical processes of drugs. Besides, it contributed to drug modification and exploitation. With the development of new instruments and utilization of drug analytical techniques, researches on drug metabolism have become more and more convenient. Developing and improving methods that can be used in the analysis of drugs and their metabolites in organism could provide strong tools for drug assay and new drug exploitation.Obviously, capillary electrophoresis (CE) has many distinctive advantages when it is used to analyze drugs and their metabolites in organism, such as high resolution, low sample consumption, simple pretreatment procedure and so forth. However, the inner diameter in the capillary column is often only around 100 micrometer. Due to the short optical path, when CE is coupled with spectrometry, its detect sensitivity is not as satisfied as that of high performance liquid chromatography (HPLC). So CE is seldom seen in practical applications. The method of CE coupled with chemiluminescence (CL) was set up in this dissertation to analyze very low concentration drugs in organism. The coexistent components that have no CL response will not influence the analytical result.In the first part of this dissertation, basic theories of CE and CL were introduced, focusing on interfaces between CE and CL and the application of on-line CE coupled with CL for pharmaceutical analysis. Furthermore, this method was used to analyze several kinds of pharmaceutics and a drug metabolite. The main contents of the study are mentioned as below:1. The fabrication and functional improvement of the instrument of CE coupled with CL. A new CE-CL instrument was set up in the lab. For the purpose of investigating and improving this method, it was utilized in the analysis of pazufloxacin mesilate sodium chloride (PZLX) and gatifloxacin (GTLX). Within PZLX or GTLX in the running buffer, the CL signal of luminol-H2O2 was restricted distinctly. According to the relationship between intensity of the CL signal districted and the concentration of the two medicines respectively, their concentrations in pharmaceutics were obtained.