Detection Of Methylation Of The P16 And P15 Gene Promoter In Hepatocellular Carcinoma

Objective To detect methylation of the pl6 and pl5 gene promoter in 30 hepatocellular carcinoma patients ,and to study the association of the presence pl6 and pl5 gene promoter methylation with hepatocellular carcinogenesis.Methods We designed the primers to discriminate between methylated and unmethylated alletes following bisulfite treatment.The methylation status of pl6 and pl5 gene were evaluated with the method of methylation specific polymerase chain reaction(MSP) in 30 HCC and 5 normal liver tissues.The data were statistically analyzed.Results Methylation of the pl6 and pl5 promoter were detected in HCC(53.3%, 16/30, P < 0.05;46.7%,14/30, P > 0.05.respectively ) ,and methylation were not detected in normal liver tissues. Methylation of the p16 and pl5 promoter were found in the same tissue of 43.3%(13/30) HCC, there was an associativity (Pearson coefficient correlation=0.741,P<0.01) and consistency (x~2=0.25,P>0.05) between pl6 and pl5 gene promoter methylation during hepatocellular carcinogenesis.A significant correlation was not found between methylation and clincopathological features, and HBsAg in HCC (P>0.05) .Conclusion There was a high frequency of aberrant methylation of P16 and pl5 gene promoter, and it may play a vital role in heptocarcinogenesis.