Study Of Immune Function In Obesity

Objective: To study the immune function in obese subjects through the detection of immune cells which participate in cell-mediated immunity, humoral immunity and nonspecific immunity in peripheral blood, and analyze all the factors that may influence the immune function.Method:T-lymphocyte subsets, nature killer cells, B cells were examined by BECKMAN COULTER EPICS XL Flow Cytometer. Leukocyte, lymphocyte, granulocyte and monocyte were examined by SYSMEX SF3000 Automatic blood analyzer. Blood glucose, triglycerides and cholesterol were examined by OLYMPUS AU5400 fully automatic biochemistry instrument. Statistical analyses were performed using Statistical Packages for Social Science (SPSS) version 13.0 statistical software.Results:1. The obese subjects had obviously higher leukocyte, granulocyte, lymphocyte count whereas the percentage of lymphocyte were lower.2. CD3~+, CD4~+ lymphocyte count and the CD4~+/CD8~+ decreased in obese subjects, There were 22 obesity whose CD4~+/CD8~+ was less than 1, and NK cell elevated in obese subject. No significant difference was found in CD8~+T cell and B cell.3. obese subjects were divided into two groups by CD4~+/CD8~+, the patients which CD4~+/CD8~+ were <1 had higher BMI, glucose than the patients which CD4~+/CD8~+ were>1.