Analysis Of Genotyping And Antifungal Susceptibilities Of Clinical Isolates Of Dermatophytes From Children

Objective â‘ To investigate the DNA type of clinical isolates of dermatophytes from children using RAPD, and to study the relationship between genotypes and the sites of infection of dermatophytes.â‘¡ A modified M38 - P scheme was used in evaluating the antifungal susceptibility of clinical isolates, and to investigate the relationship between DNA type and antifungal susceptibility.Methods To collect the specimens of skin and nail scrapings ,hairs from dermatophytosis under twelve years. Microscopic examination and fungal cultures were made and all strains were morphologically identified. The genotypical analysis was performed using the random amplification of polymorphic DNA (RAPD) method, the primers used were from literatures, the sequences of the four primers were 5'-ACCCGACCTG-3', 5'-GGTGACGCAG-3', 5'-GGTGCGGGAA-3', 5'-CCCGTCAGCA-3',respectively. The antifungal susceptibility study was conducted using the microdilution test , eight antifungal agents including Fluconazole , Itraconazole, Terbinafine, Ketoconazole, Liranaftate. Butenafine. Econazole and Bifonazole were tested..Results â‘  Thirty-five dermatophytes were isolated from children:25 Trichophyton rubrum (71.4% ) was isolated from 12 cases of tinea corporis, 6 cases of onychomycosis,4 cases of tinea pedis and 3 cases of tinea cruris; 4 Trichophyton mentagrophytes(11.4%) and 4 Microsporum canis.{11.4%) were isolated from 2 cases of tinea capitis and 2 cases of tinea corporis respectively;2 Trichophyton tousurans...