ζ Chain Level Of T And NK Cells In Patients With Lymphoma And Its Clinical Significance

Objective:To study the ζ chain level of T and NK cells in peripheral blood of patients with lymphoma and investigate the relationship between the ζ chain level and tumor occurrence progression and its clinical effect in lymphoma.Methods:The peripheral blood mononuclcear cells in 39 patients with lymphoma and 30 healthy blood donors were examined to the ζ chain MFI of T and NK cells by flowcytometry with fluorescent anti CD3(for T cells), anti ζ chain and anti mouse IgGγ monoclonal antibodies.Results: (1) The ζ chain expression of NK cell were stronger than T cells which suggested the higher ζ chain protein level of NK cells' membrane.(2) T and NK cell ζ chain levels in 82.1 %(32/39) and 79.5%(31/39) of the lymphoma patients were lower than the healthy control , respectively .There was a significant difference inζ chain expression levels of T and NK cell between the lymphoma patients and the healthy control (p<0.05).(3) There was a significant difference in ζ chain expression of T cells and NK cells in stage I, II and III, IV lymphoma(P<0.01 ).The C chain expression of T cells in stage I, II and III, IV decreased to 93% and 43.1% ;that of NK cells in stage I, II and III , IV decreased to 94.2% and 47.7% of the healthy control level, respectively .(4) After the regular treatment, there was a significant difference in ζ chain expression levels of T and NK cells between CR and PR lymphoma patients (P<0.01) .(5) There was a significant correlation in ζ chain expression between T and NK cells (P<0.01) ,r=0.661.Conclusion: Reduced T and NK cells ζ chain levels of the lymphoma patients are gemerally consistent with tumor progression and correlated with clinical effect. There was a significant correlation in ζ chain expression between T and NK cells. The ζ chain level of T and NK cells is related to the mechanism of immune escape, and tumor origination, progression and its clinical effect in lymphoma.