Shock Wave Permeabilization With Adriamycin Into T24 Cells

Objective:Superficial bladder cancer is the most common tumor in the urology department of china. Intravesical instillation after TUR was adopted to prevent recurrence and development clinically. But some tumors don't respond to drugs. It has previously been confirmed that shock wave can cause a transient increase of the permeability of the tumor cell membrane and enhance chemotherapeutic effects of anticancer drugs. We therefore hypothesized that shock waves might be able to transfer anticancer drugs into bladder tumor cells and examined this in vitro. It aims to look for a new approach for improving bladder chemotherapeutic effects.Methods:In this study, the T24 cell suspensions containing 1×106cells/ml and 5μg adriamycin /ml was exposed to shock waves(16KV,300), generated with the lithotriptor MZ.ESWL-Ⅵ. Changes of the cellular membrane permeability were also studied by measuring the intracellular concentration of adriamycin before and after shock wave exposure. Simultaneously, we examined the activity of ATPase on the cell membrane signifying tumr cellular damage and the amount of malonaldehyde(MDA) in the cytoplasm., a measure of lipid peroxidation and free-radical activity.Results:Compared with the control group, the fluorescence intensity of adriamycin in the shock wave-treated T24 cells was 1.7-fold higher than that of control. And the amount of malondialdehyde (MDA) greatly increased. But no difference was noted in the activity of ATPase on the cell membrance between the groups.Conclusion:In conclusion, shock waves increased the permeability of T24 cell membrane and led to the accumulation of adriamycin in T24 cells. And Our study suggests that this combination therapy may be effective in some tumor cells, which are refractory to anticancer drugs.