Study On The Immune Mechanism Of Antitumor Effect Of Decoction Of Juglans Maxim's Branch On The Tumor-Bearing Mice

Objective: To study the immunoregulation effect of DJM in mice bearing S₁₈₀ tumor cells.Further perfect the anti-tumor effect of DJM and efficacy enhancing of DJM on mice who are treated with chemical therapeutic drug like cytoxan. Methods: To evaluate the drug's activity of anti-tumor and judge DJM's efficacy enhancing by calculating the ratio of anti-tumor and q. Applying ELISA method to measure the level of IL-2 and IL-4 in serum, while we can observe the influence of DJM acting on the cytokine. To measure T cell phenotype by FACS method and to observe the influence of DJM acting on the CD4, CD8 molecule. The protein expression of p53 was evaluated by SABC immunohistochemistry. Results: â‘ The ratio of anti-tumor was 35.68%.The compounds of DJM and CTX can enhance the anti-tumor effect(q=0.867),the compound's anti-tumor effect enhanced obviously (the ratio of anti-tumor was 53.41%);â‘¡DJM can increase the level of IL-2, but decrease the level of IL-4;â‘¢DJM enhanced the expression of the CD4 molecule and reduced the expression of the CD8 molecule;â‘£DJM group and model group showed the conspicuous difference in the positive expression of mt-p53 and DJM can reduce the expression of mt-p53. Conclusions: DJM has efficacy enhancing on S₁₈₀ sarcoma mice who are treated with the anti-tumor drug like cytoxan. DJM can regulate cytokine to correct the phenomenon of Th1/ Th2 drift in tumor mice ,maintain Th1 of advantage appearance and correct the disbalance of CD₄⁺/CD₈⁺ to normal.The immunosuppressive state of the tumor mice was regulated.DJM can also regulate the expression of mt-p53 gene to show the anti-tumor function.