The Changes Of Myocardial Metabolism And Cytokine Of Hypertensive Diabetic Rats And The Protection Of L-CN

Objective: Try to explore the influence of diabetes on the heart function of hypertension rats from energy metabolism and inflammatory reaction aspects and to observe the heart conservation mechanism of L-CN on hypertensive-diabetes rats. Methods: Through 2K1C hypertensive rat model and STZ (60mg/kg) induced diabetes model, the model of hypertensive-diabetes rat was developed. SD rats were divided into five groups: Sham-operated group (SO+NS);hypertension control group (HBP+NS); hypertension L-CN group (HBP+LCN); hypertensive-diabetes control group (HBP-DM+NS) ; hypertensive-diabetes L-CN group (HBP-DM+L-CN).The treated groups were given L-CN (100mg/kg) through intragastric administration. The control group was given the same volume of normal sodium, 1/d, continue for 10 weeks. After 10 weeks, the rats were given echocardiogram, hemodynamics check, and store the blood preparation for detect GLU, TCH, TG, HDL-C, LDL-C, FFA, AngII, ET-1, TNF-a, then executed the rats, calculated the cardiac index (CI). The cardiac muscular tissues were made for HE drum dyeing, and to observe ultrastructural organization through electron microscope. Results: HBP-DM group compared with HBP group, CI rised obviously, EF, FS decrease manifestly, LVSP decrease manifestly, LVEDP rised obviously, TC, LDL-C rised obviously, HDL-C decrease manifestly, FFA rised obviously (p<0.05); Cardiac muscle HE drum dyeing and ultrastructural organization observation showed that pathological changes is more severe in HBP-DM group than in HBP group. The level of AngII, ET-1 and TNF-a are higher in HBP-DM group than in HBP group (P<0.05). L-CN group compared with NS group, the above changes all relieved, and the CI, EF, FS, LVSP, LVEDP, LDL-C, FFA, AngII, ET-1, TNF-a relieved significantly. (p<0.05)。 Conclusion: Diabetes can aggravate the myocardial hypertrophy and cardia insufficiency progress of the hypertensive rat. The mechanism maybe related with the diabetes aggravate myocardial metabolism and inflammatory reaction degree of the hypertensive rat. L-CN can balance the cadiocyte energy metabolism disorder, protect the heart function and decrease the release of inflammatory factors, so it can soften the hazard of diabetes on cardiovascular system.