Recovery Of Adult Rat Spinal Cord Injury By Co-transplantion Of Human Embryonic OECs And NSCs

Background: Few survivable injuries compare with the devastating physical and psychological consequences of the paralysis that results from trauma to the spinal cord.It influenced the normal life of the suffers severely. Clearly,therapies to enhance neurological function after spinal cord injury are urgently needly, but it still remained undiscovered. Although the inhibitory environment of the CNS has been recognized for nearly a century ,not until the past several decades have CNS myelin and glial scar become appreciated as primary blockades to CNS regeration after injury and that's why the clinical symptom occurs. NSCs are undifferentiated nervous system cells that are capable of proliferation, repeated subculture, and differentiation into all the major cell types of the adult central nervous system, including neuron, astrocyte and oligodendrocyte. Therefore it may be a promising method for the therapy of SCI to transplant the genetic engineering NSCs modified to secrete neurotrophic factors. Olfactory ensheathing cell has the characteristics of CNS and PNS。Grafts of neural stem cells could secrete many types of cellular adherent moleculars and neurotropin factors and these substances could support host axonal growth greatly. In our study we explored the feasibility to graft the combined hOECs and hNSCs into the contused spinal cord and compared the functional recovery with that of co-transplantation by hOECs and hNSCs separately. We aim to examine the potential effect of co-transplantation on spinal cord injury and provide an idea for experimental and clinical research on therapy of spinal cord injury.PartⅠExperimental study of the culture of human embryonic olfactory ensheathing cells in vitroObjective To investigate the isolation and culture methods of human embryonic olfactory ensheathing cells in vitro in preliminary. Methods hOECs were harvested from three to five months old Abortus based on delaying differing rates of various cell types and...