The Study On The Relationgship Between Hepatitis B Virus-associated Glomerulonephritis And Human Leucocyte Antigen DR Allele

[Objective]: To find the susceptible and/or the resistant genes correlated with hepatitis B virus-associated glomerulonephritis (HBV-GN) and determine the immunogenetic basis for the development of HBV-GN by analysis of the relationship between HBV-GN and the polymorphism of human leucocyte antigen (HLA) DR allele.[Methods]: 30 patients finally diagnosed with HBV-GN by clinical, laboratory and renal pathological examination were chosen as the study subjects. These patients were differentiated in two subsections of membranous nephropathy (MN) and membranoproliferative glomeru- lonephritis(MPGN). 30 individuals chronically infected by HBV without liver and renal involvement were chosen as HBV controls and 100 healthy individuals without blood relationship from the same population chosen as normal controls. HLA-DR genotypes were determined by using DNA chip technique in the above-mentioned subjects. HLA-DR allele frequency discrepancies were compared one by one among the study group and the control groups after counting the frequency of each HLA-DR allele of each group. [Results]:1. The most frequent genotype in the normal controls was HLA-DRB1*09, whose positive rate was 18.0%, the next were HLA-DRB1*12(17.5%) and HLA-DRB1*04(12.0%). The frequency of HLA-DRB1*15 allele was 18.3% in the HBV controls and HLA-DRB1*03(serology equivalent is DR17) allele was 15.0% in the patients with HBV-GN, which were also higher in the two groups...