Structural Characterization And Chemical Modification Of Polysaccharides From Acanthopanax Senticosus

Polysaccharide from Acanthopanax senticosus, which is a costful traditional medicinal plant, is a kind of active compounds as well as glycoside in Acanthopanax senticosus, and it has many pharmaceutical activities, such as anticancer, enhancing immunity of human body. In this paper, four kinds of products were extracted from roots using different kinds of separation and purification methods. They were cool alkali extracted water-soluble product (named ASC-1), acid extracted water-insoluble product(named ASC-2), hot alkali extracted water-soluble product (named ASH-1), hot alkali extracted water-insoluble product (named ASH-2), acid extracted water-soluble product(named ASA-1), acid extracted water-insoluble product(named ASC-2). The primary structure of two kind of acid extracted water-soluble product named ASA-1P3A and ASA-1P3B were determined by chemical and modern instruments analysis techniques. The product of ASC-2 was modified by chemical methods.The roots of Acanthopanax senticosus was extracted by ethanol, water, cool alkali, hot alkali and acid in turn, and the products of ASC-1,ASH-1, ASA-1 were studied. It was proved that the purified product of ASC-1, named ASC-1P2B, was only composed by xylose. The composition of ASH-1P3 (the purified ASH-1) was complex, including rhamnose, arabalose, xylose, mannose, glucose and galactose.ASA-1 was further purified by Q sepharose F.F. and Sephacryl S-100 HR, and two kinds of pure polysaccharide ASA-1P3A and ASA-1P3B were acquired and were further studied their structures. They were proved to be higher purities by gel permeation chromatography. The molecular weight of ASA-1P3A and ASA-1P3B were estimated as 38.287 kD and 7.228 kD respectively by HPGPC. The sugar content were 98.31% and 97.07% respectively and the uronic acid content were 29.70% and 30.80%. The primary structure of ASA-1P3A and ASA-1P3B were elucidated respectively by chemical methods and spectroscopic techniques, such as acid hydrolysis , periodate oxidation, Smith degradation, methylation, GC, HPLC, IR, UV, 1D-NMR, 2...