A Primary Experimental Studies On A Propolis-complex Desensitizer Of Dentin Hypersensitivity Developed By Ourselves

Objective: The primary aim of this study was to identify major element of this self-made desensitizer. The secondary aim was to evaluate the biocompatibility of the desensitizer, and studied the effect of the desensitizer on dentinal tubules in preparation for its next studies and clinical use.Methods: Identify major element of this self-made desensitizer by X-ray diffraction analysis. According to the relative Chinese national standards, the oral mucous membrane irritation response of this desensitizer was evaluated using rabbits, the short term systemic toxicity test: oral route response of this desensitizer was evaluated using rats; the pulp and dentine usage test response of this desensitizer was evaluated using dogs. The models of dentin hypersensitivity were made on tooth of dogs and fresh bicuspids of human, the morphological changes of each specimen were observed with a scanning electronic microscope (SEM).Results: After X-ray diffraction analysis, major element of this self-made desensitizer including K, F, Mg, Na, Si, etc. The results showed the local or systemic abnormal response on rabbits including histopathological findings was not observed in the oral mucous membrane irritation test; the abnormal response on rats was not observed in the short term systemic toxicity test: oral route; the exponent of inflammatory reaction of pulp was between the negative group and positive group in the pulp and dentine usage test. The observations of SEM showed that the dentinal tubules were obstructed.