Studies On Processing Technology And Quality Standard Research Of The Herb Of Cirsii

The subject of Studies on Processing Technology and Quality Standard Research of herb of herba Cirsii was the subsidiary subject of a national "ten-five" major technology project-Standardization research of 50 kinds of traditional Chinese medical herb in pieces. The author studied systematically and deeply the processing history, contemporary documents, effective contents, standardized processing technology, quality standard and fingerprint of herb of herba Cirsii, which was often used in clinical research.The clinical practice has proved that herb of herba Cirsii has significant effect in cooling the blood and stop the bleeding and promoting the subsidence of swelling.The herba Cirsii are used in Chinese herbal medicine to treat constipation and improve visual acuity. Its chemical ingredients include flavonoids alkaloids organic acids. The main ingredients of it are flavonoids and organic acids.Based on the theory of traditional Chinese medicine, we demonstrated the effective part of herb of herba Cirsii and herb of herba Cirsii carbonisatus through the chemical separation and pharmacodynamical screening as flavonoids .The thesis determined the total contents of flavonoids in herb of herba Cirsii carbonisatus and linarinin herb of herba Cirsii carbonisatus by spectrophotometry and HPLC.With the study of the processing history and contemporary documents, indicated by contents of linarin and water, the optimal processing technology with water was identified with L₉(3)⁴ orthogonal design, the factors of which were drying time and drying temperature used separately. The technology parameters were described as follows: pick out the impurity and clean quickly, then dry at 80℃ for 1 hour. Indicated by contents of total flavonoids, the optimal stirring technology was identified with L₉(3)⁴ orthogonal design, the factors of which were stirring time and stirring temperature used separately. The technology parameters were described as follows: stir at 260℃ for 5 min.The thesis also carried out quality standard research of the herb of herba Cirsii and herb of herba Cirsii carbonisatus.Based on principles of fingerprint chromatography of Chinese herbal medicine, the optimal extraction method for herb of herba Cirsii and its corresponding HPLC-based fingerprint chromatography have been established through HPLC gradient elution. By means of similarity calculation and main ingredients analysis with fingerprint chromatography software, the common pattern of the herb of herba Cirsii s was established. The thesis also compared the similar degree of the herb of herba Cirsii from different distinct and the fingerprint chromatography of the herb of herba Cirsii and processed product of it.