| Objective To investigate the effects of benzalkonium chloride and timolol maleate with different concentraion on cell's damage and the induction of the inflammatory mediators by Culturing human lens epithelial cells (SRA01/04).Method Cells from a human lens epithelial cell line(SRA01/04) were cultured in vitro till confluenced. Then, dispersed the cells with trypsogen and transfered the cells into 6-well-plates. The amounts of benzalkonium chloride (0.01%) and timolol(0.5%) used in eyedrops were diluted into different concentrations: benzalkonium chloride were diluted into × 100 to × 1000 dilutions while timolol into ×10 to ×100 dilutions. Then, durgs with different concentrations were added into medium. After 48 hours Culturing, cell's morphological changes were observed and cell-free culture supernatants were...
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