Structural Shifts In Gut Microbiota Of Rats In Response To Bile Duct Ligation

Obstructive jaundice(OJ)is a clinical symptom of multi-systematic malfunction due to biliary obstruction which is caused by mechanical factors on intra- and extrahepatic bile duct. OJ and its complications are highly associated with intestinal microbiota. The bile duct ligation (BDL) of SD rats is the most common model for experimental study of obstructive juandice. We focused on the analysis of gut microflora in BDL rats. The structural shifts of bacterial community in gut of the BDL group and sham operated (SO) group were compared. The fecal samples were collected from both groups at 3 days before and 3, 7, 14 days after operation, respectively. The total fecal microbial DNA was extracted. For molecular analysis of the intestinal bacterial community, ERIC-PCR, 16S rRNA gene V3 region-DGGE and group-specific DGGE (for four groups- Lactobacillus spp., Clostridium leptum subgroup, Bifidobacteria and Bacteroides spp., respectively) were used and the principal component analysis (PCA) was applied.The ERIC-PCR electrophoresis showed that there were individual...