The Expression Of SSTR_2A,SSTR₅ And CgA,Syn,PR In NSCLC And Explore Their Relationship And Clinical Significance

Background:There is often with neuroendocrine(NE) differentiation in non—small cell lung cancer (NSCLC). It's an important manifestation of heterogeneity of lung cancer. And non—small cell lung cancer with neuroendocrine differentiation (NSCLC—NE) has its particular biological features. Somastostatin Receptors (SSTRs) have five different subtypes, SSTR1.5. SSTR2 have two isomers, sstr_2A and sstr_2B. In the past, individual expression patterns of sstr subtypes were based on methods such as in situ hybridisation and polymerase chain reaction at the transcriptional level, so their cellular localisation, distribution, and expression pattern is not clear.Somatostatin receptors (SSTRs) were frequently identified in human normal tissues and neuroendocrine tumors, and have been detected in non-neuroendocrine tumors, such as human breast cancer and cancer of colon, but their expression in NSCLC has been seldom reported in large quantity. It has been identified that NSCLC contains Progesterone Receptor(PR), but it's relationship with SSTRs is seldom reported. At present it has been seldom reported the expression and subcellular distribution of SSTRs in NSCLC and their relation with neuroendocrine differentiation, clinical and pathology.Objective:To investigate the expression of Chromgranin A, Synaptophysin, Progesterone Receptor and Somastostatin Receptor 2A,5 in NSCLC and explore the correlation and the relationship of between each two index, and the clinical significance of their expression in NSCLC.Methods:A total of 85 NSCLC tissues, 15 pericancerous normal pancreatic tissues were collected.The Chromgranin A, Synaptophysin, Progesterone Receptor and Somastostatin Receptor 2A,5 expression was investigated by immunohistochemical staining(S-P method) with a specific antibody. Statistical analysis was done by SPSS 13.0, P<0.05 were considered significant.Results:The positive rate of SSTR2A expression in NSCLC was 69. 4% (59/85). The positive rate of SSTR5 expression was 64. 7% (55/85) in patients. SSTR2A is mainly located in cellular membrane, seldom stained in cytoplasm. SSTR5 is mainly located in cellular membarane and cytoplasm. The positive rate of SSTR2A and SSTR5 were related to TNM stage and lymphnode metastasis (P<0. 05), but not to other clinical characteristics of NSCLC(P >0. 05). The survival curve of SSTR(SSTR2A,SSTR5) negative group is obviously lower than SSTR(SSTR2A,SSTR5) positive group(P<0. 05). The positive rate of SSTR_2A and SSTR₅ expression was 2/15 and 3/15 in normal tissues nearby the cancer tissues. There was significant difference in expression of SSTR(SSTR2A,SSTR5) between normal tissues nearby the cancer tissues and cancer tissues (P<0. 05) . The positive rate of CgA and Syn expression is 1/15 in normal tissues nearby the cancer tissues. The positive rate of SSTR2A expression in NSCLC was 21. 18%(18/85). The positive rate of Syn expression was 34.12% (29/85) in patients. There are 7 samples positive for both of the two indexes, the positive rate is 8. 24%(7/85). There was significant difference in expression of Syn between normal tissues nearby the cancer tissues and cancer tissues (P<0. 05) . According to the standardization of neuroendocrine differentiation(NE) in this trial, there are 25 cases with NE, its positive rate is 29. 41%.. The positive rate of CgA and Syn were related to lymphnode metastasis(P < 0.05), but not to other clinical characteristics of NSCLC(P>0. 05). The positive rate of Syn was related to TNM stage (P<0.05). The positive rate of NE were related to TNM stage and lymphnode metastasis(P < 0.05),but not to other clinical characteristics of NSCLC(P>0. 05). The survival curve of Syn negative group is obviously lower than Syn positive group(P<0. 05). All the 15 normal tissues nearby the cancer tissues are negative of PR. The positive rate of PR expression in NSCLC was 57.65% (49/85) . Adencarcinoma is 71.11%(32/45),obviously higher than squamous cell carcinoma(42. 42%, 14/33 )and adenosquamous carcinoma(42. 86%, 3/7) , P<0. 05o There is significant dependability between Syn and SSTR2A,SSTR5 in NSCLC.Conclusion:The expression of SSTR2A and SSTR5 suggests good prognosis and long survival(P < 0.05).The expression of NE has no relationship with prognosis.NSCLC with the expression of PR may be hormone-dependent tumor, especially the adenocarcinoma of lung. There is partial instruction value for the therapy of lung cancer. There is significant dependability between Syn and SSTR2A,SSTR5 in NSCLC.