| During the long period of researching and practicing in medicine , a lot of ways of research have been set up, and the animal model is one of the ways . The animal model which is set up for the research of Chinese traditional medicine is used for reproducing the character of anthropometric physiology and disease. Being the beginning of the experiment research , most of the animal models reflect the whole appearance and the essence of syndrome difficultly , and with the character of fuzziness;simplicity; instability. So in the course of exisiting research of traditional Chinese medicine, most animal models are duplicated by medical doctor etiological factor and pathology in most animal experiment ,but the emphasis of the way is not so much syndrome as disease , thus it is difficult to reflect the therapeutic principle of determining the treatment based on differentiation of symptoms and signs and the character of traditional Chinese medicine curatio. Currently there is no unified experimental animal model of syndrome and disease , so how to bind the syndrome of Chinese traditional medicine and disease and built the unified experimental animal model of syndrome and disease to make it be reproducibility and practicability are the technological key of traditional Chinese medicine pharmacology. The destination of the topic is to discuss the relativity of the singlet Acute Myocardial Infarction in rats and the Acute Myocardial Infarction Model with Blood Stasis in Rats and to establish the foundation for evaluate the experimental animal model of the research of traditional Chinese medicine pharmacology scientifically.There should be important direction sense and application value through the exploitation of the new traditional Chinese medicine .0.05% adrenalin hydrochloride;epinephrine hydrochloride is injected to Wistar rat subcutaneously two times interval four hours in the experiment . two hours after the first injection ,the rat is made to swim for five minitues in the four centi-degree aqua astricta ,then take out of the rat and dry it, and put it into the breeding cage . Establishment the model of the Acute myocardial infarction in rats by hypodesis aeteria coronaria after twenty-four hours. Collect the blood or measure the indexes of haemodynamics after twenty-four hours again. Western medicine Nitroglycerin and traditional Chinese medicine sowthistle-leaf ixeris seedling are applied to treat the Acute Myocardial Infarction Model with Blood Stasis in Rats and the singlet acute myocardial infarction in rats and compare the difference of them..Half animoals of each group under the state of chloral hydrate anesthesia are recorded the LVSP. LVEDP.±dp/dtmax. SBP. DBP by RM-6000 polygraph.Then the ECG including the HR are measured in rats.Blood is collected through piping from abdominal aorta. Activities of CK.AST.LDH of blood serum are measured by COBAS-FARA automatic biochemistry analyzer. Activities of FFA. SOD. MDA. GSH-PX. CK-MB. LDH.AST.NO of blood serum are measured in the way of kit. Activities of PGI2. TXA2. ET. TNF-αare measured by radioactive immune.Myocardium of left ventricle are dyed in the N-BT phosphate buffer, normal tissues were dyed while ischemic tissues were not. Slice ischemic myocardium are weighed.MIS was expressed as the ratio ofischemic myocardium weight to left ventricle wet weight. Half animals of each group are measured some indexes of hemorheology, including the blood plate adhesive rate. the blood plate aggregate rate. extracorporeal thromb .blood sedimentation. PRBC . elastic graph of thromb . The reason of the indexes measured lie in the abnormality of the blood biochemistry, haemodynamics, viscosity of blood after the the Acute Myocardial Infarction, and the changes intensified the extent of the Acute Myocardial Infarction ,thus, reviewing the result of the indexes of the Acute Myocardial Infarction can act as an objective index of evaluating the model effect .According the moden medical science , metabolic effect, hypoxia, blood stasis, blood circulation disorder,.the abnormality of blood flow character and viscosity result from all kinds of causative agent the whole or part of tissue and organ will induce the whole tissue and organ hydrops; inflamm development; thromb formation;tissue degeneration; desmoplasia and other kind of pathological change .All of them are recapitulated in the pathology essence of blood stasis.The reason that pathological change result from blood stasis correlate with Myocardial Infarction dependently .Weather or not the Acute Myocardial Infarction Model with Blood Stasis intensified the extent of singlet Acute Myocardial Infarction and it is appropriate to duplicate the animal model guidanced by the cause and pathology of medical science in pharmaco-research of traditional Chinese medicine are the destination of our research..The results of the experiment show that :①Both the singlet Acute Myocardial Infarction in rats and the Acute Myocardial Infarction Model with Blood Stasis in Rats can induce theelevated tendencyof index of MIS,AST,CK,LDH ,there is no obvious difference of them. There is also no obvious difference of the depressed extent of MIS,AST,CK,LDH when we compare the Nitroglycerin and the sowthistle-leaf ixeris seedling as the intervention .It is no difficult to conclude that there is at equal generally of the change of the Myocardial morphology and the AST,CK,LDH as the criterion of the Acute Myocardial Infarction.②Both the singlet Acute Myocardial Infarction in rats and the Acute Myocardial Infarction Model with Blood Stasis in Rats can induce the elevated tendencyof index of MDA,FFA,TXA2,TNF-α,while can induce the depressed tendency of index of NO,PGI2,SOD,GSH-Px.The change extent of the latter is more deeply than the former. There is no obvious difference of the depressed tendency of index of MDA,FFA,TXA2,TNF-αand the elevated tendencyof NO,PGI2,SOD,GSH-Px . It is no difficult to conclude that there is at equal generally of the change of indexes mentioned upwards when we compare the Acute Myocardial Infarction Model with Blood Stasis in Rats to the singlet Acute Myocardial Infarction in rats ..③Both the singlet Acute Myocardial Infarction in rats and the Acute Myocardial Infarction Model with Blood Stasis in Rats can induce the elevated tendencyof index of blood plate adhesive rate, the blood plate aggregate rate, the weight and the length of extracorporeal thromb, blood sedimentation, PRBC, the value of ma of elastic graph of thromb,while can induce the depressed tendency of r.k of elastic graph of thromb.The change extent of the latter is more deeply than the former. Thereis no obvious difference of the depressed tendency of index of blood plate adhesive rate, the blood plate aggregate rate, the weight and the length of extracorporeal thromb, blood sedimentation, PRBC, the value of ma of elastic graph of thromb and the elevated tendencyof r.k of elastic graph of thromb.It is no difficult to conclude that there is at equal generally of the change of indexes mentioned upwards when we compare the Acute Myocardial Infarction Model with Blood Stasis in Rats to the singlet Acute Myocardial Infarction in rats ..④Both the singlet Acute Myocardial Infarction in rats and the Acute Myocardial Infarction Model with Blood Stasis in Rats can induce the depressed tendency of index of HR,while can induce the elevated tendency of ST and T wave.The change extent of the latter is more deeply than the former. There is no obvious difference of the elevated tendency of HR and the depressed tendencyof ST and T wave .It is no difficult to conclude that there is at equal generally of the change of indexes mentioned upwards when we compare the Acute Myocardial Infarction Model with Blood Stasis in Rats to the singlet Acute Myocardial Infarction in rats ..⑤Both the singlet Acute Myocardial Infarction in rats and the Acute Myocardial Infarction Model with Blood Stasis in Rats can induce the elevated tendency of LVEDP,while can induce the depressed tendency of SBP.DBP. MAP. LVSP.±dp/dtmax.The change extent of the latter is more deeply than the former. There is no obvious difference of the depressed tendency of LVEDP and the elevated tendency of SBP.DBP. MAP. LVSP.±dp/dtmax .It is no difficult to conclude that there is at equalgenerally of the change of indexes mentioned upwards when we compare the Acute Myocardial Infarction Model with Blood Stasis in Rats to the singlet Acute Myocardial Infarction in rats ..In a word , althouth the change extent of the myocardial morphology, biochemistry of blood , hemorheology , haemodynamics and ECG is more deeply when we compare Blood Stasis Acute Myocardial Infarction model than singlet Acute Myocardial Infarction model. But there is no obvious difference between them ,and it is the same result as the intervention of Nitroglycerin and the sowthistle-leaf ixeris seedling.It can be infered that when we evaluate Acute Myocardial Infarction with Blood Stasis we can build singlet Acute Myocardial Infarction instead of Acute Myocardial Infarction Model with Blood Stasis. |
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