Le-Mai Granule Decreases The Neointima Hyperplasia And Promotes The Stem Cell Mobilization In The Artery Endothelial Injury Rabbits

Objective: The mechanical injury of arterial endothelium may cause proliferation of vascular smooth muscle cells (VSMCs) and lead to hyperplasia of neointima, which has been considered as the principal reason of restenosis after percutaneous tranluminal coronary angioplasty (PTCA) or stent implantation. The endothelial progenitor cells (EPCs) mobilization is suggested as an important way for proving the repairment of endothelial injury. Some cytokines, such as P-selectin, cellular adhesion molecule (ICAM-1) and plasma fibrinogen, are involved into this process. Le-Mai granule, a kind of traditional Chinese medicine, has been used to treat coronary heart disease effectively. The aim of this study is to evaluate whether Le-Mai granule can decrease the hyperplasia of neointima of injured artery and decline its possible mechanisms, especially effection of it on the EPCs mobilization.Methods: 1. New Zealand white rabbits were randomly divided into 4 groups: sham operated control group(SC), sham operated treatment group(ST), injured artery control group(IC), and its treatment group(IT). 2. The endothelium of abdominal aorta was injured by balloon to establish the endothelial injury model, and the rabbits only with ligating right femoral arteries as SC groups. The SC groups were observed for 7 days, and the endothelial injury groups for 28 days. IT groups were fed Le-Mai granule (2.5gkg^-1·d^-1) from the day before operation to the day before sacrifice. 3. Investigation parameters included: (1) Blood concentrations of fibrinogen, P-selectin and sICAM-1; percentage of CD34-positive cell at the 7^th, 14^th and 28^th day after operation . (2) Neointima hyperplasia parameter: the injured sections of abdominal aorta were harvested to...