The Effect Of Sanshengyin On The Expression Of Bcl-2 And Bax Protein In The Rats After Focal Cerebral Ischemia

Abstract: Background Stroke is the third-leading cause of death in China. Currently, tissue plasminogen activator (tPA) is the only approved treatment used to reduced the disability of patients with acute ischemic stroke, but this compound has a very narrow therapeutic window of 3 h and the extent of its protective effects are limited. Evidence suggests that apoptosis cell death contributes to the progression of tissue damage following cerebral ischemia. Protective effects of traditional Chinese medicines have been widely reported. San Shengyin decoction is reported to be Calcium antagonist after stroke. To investigate the effect of SanShengYin in the rats after focal cerebral ischemia is helpful to study herbs ischemia antagonist mechanism.Objective: To investigate the effect of SanShengYin on the expression of Bcl-2 and Bax protein in the rats after focal cerebral ischemia.Methods: The model of focal cerebral ischemia and reperfusion was induced. The Sprage-Dawley rats were dividedrandomly into two groups. The first group is used to estimate the volume of the infarction with the TTC method; the second group is used to analyze the expression of Bcl-2 and Bax positive cells at 3h,6h,12h,24h,3d after reperfusion with Immunohistochemistry method.Results: Compared with the results of the MCAO group, the degree of cerebral ischemia is lightly; infarct volume is decreased; Bcl-2 protein positive cells were increasing. But Bax protein positive cells were not decreasing, the results of the two group have no significant differentiation (p>0.05).Conclusions: SanShengYin can protect nerve cell by increasing the expression of Bcl-2 protein,when the cerebral ischemia reperfusion injury occurring.