Experiment Study Of Bombesin On Hepatical Protection In Rats With Obstructive Jaundice

Objective To evaluate the effect of bombesin (BBS)on hepatical protection in rats with obstructive jaundice. MethodsForty male Wistar rats were randomly divided into four groups: control(A), sham operated (B), bile duct ligated (C), BDL+bombesin (D).On day 10, serum ALT, BIL-T and endotoxin level were determined ininferior caval blood, also hepatic oxidative status was estimated on liverhomogenates by measurements of SOD,MDA,XOD,GSH,GST.Liver tissue samples were also examined histologically, hepatical MCP-1and TGF-β₁ were expressed by immunohistochemistry. ResultsSerum ALT,BIL-T and caval endotoxaemia were elevated in rats withobstructive jaundice. This effect accompanied by decreas of SOD,GSH and GST, increase of MDA and XOD reveals that livers were inhigh oxidative stress status. Moreover, ductus felleus epithelialproliferation and neutrophil accumulation in portal tracts weresignificantly increased, and hepatic cellular swelling could be observed.Hepatical MCP-1 and TGF-β₁ were strong positive expression inimmunohistochemistry. BBS administration reduced ALT,BIL-T andcaval endotoxaemia, decreased duetus felleus epithelial proliferation, neutrophil infiltration and hepatic cellular swelling, reduced MCP-1 andTGF-β₁ positive expression in immunohistochemistry. ConclutionsThere were neutrophil accumulation in portal tracts and high oxidativestress status in obstructive jaundice, they all damaged the liver. BBSprotected the liver against oxidative stress, decreased neutrophilinfiltration, exerts protective effects on liver in bile duct ligated rats. thisobservation may provide a novel therapeutic approach in clinic for liverinjury after obstructive jaundice.