Study Of Relationship Among Polymorphism Of Serotonin Transporter, Therapeutic Response And Adverse Effect Of Fluoxetine In Patients With MDD

Study of Relationship among Polymorphism of Serotonin Transporter, Therapeutic-Response and Adverse Effect of Fluoxetine in Patients with MDDPrefaceSSRIs antidepressant drugs are frequently used to treat major depressive disorder. This study is to investigate the relationship among genetic polymorphism of 5-hydroxytryptamine transporter gene-linked polymorphic region (5-HTTLPR), clinical response and adverse reaction of fluoxetine.MethodsThe study consisted of 89 Chinese Han outpatients diagnosed as major depressive disorder. We divided the patients into two groups according to the therapeutic effect or adverse effect and analyzed the difference of the genetic polymorphism of 5-hydroxytryptamine transporter gene-linked polymorphic region (5-HTTLPR). PCR was used to amplificate target DNA. PCR Cycling Profile: 95℃, 5min; [95℃, 30″-60℃, 30″-72℃, 1min]×35cycles; 72℃, 7min. Genetypes are divided by agarose gel electrophoresis.Statistical analysis: the SPSS 13.0 software was applied. The t-test and one way analysis of variance(ANOVA) was used in the comparison of HAMD date; the chi-square test was used in the comparison of the distribution of genotypes and allelic frequency.ResultsIn total, significant differences were found among three gene types in the comparison of HAMD date between pre-treatment and six wks post-treatment (all P＜0.005). There were significant differences of genotypic and allelic distribution of 5-HTTLPR between different effects of drug treatment (fluoxetine), the HAMD reductive date of 6 wks post-treatment were significantly different in depressive patients with different 5-HTTLPR genotype (F=3.657, P＜0.05); the frequency of genotypes and allelic distribution in patients with adverse reaction of nausea is significant different with the frequency of genotypic and allelic distribution of 5-HTTLPR in patients without this adverse reaction (both P＜0.05).ConclusionThe findings suggest that the significant clinical responses can be seen in three different gene types after 6 weeks treatment with fluoxetine; 5-HTTLPR gene polymorphism is significantly associated with clinical responses and may be associated with the adverse reaction of fluoxetine in Chinese.