Expression Of NF-κB,Survivin,c-myc And HTERT In Epithelial Canceration Of The Esophagus And Significance

Objective: Esophageal carcinoma is one of the high incidence in digestive tumors in our country, the carcinogenesis and development of which is a polygene and multiple procedure course. In the epithelial carcinogenesis of esophageal, telomerase gene and its protein educed significant effect. hTERT is the rate limited subunit of the telomerase gene, the expression of which is controlled in common by multiple factor. This experiment is to investigate the expressions and their relationships of NF-кB,survivin,c-myc and hTERT in the carcinomatous course of esophageal epithelial. The result may control the expression of hTERT, and provide theoretic basis for force-diagnose, judgement of prognosis and gene treatment of esophageal carcinoma.Methods1 The expression of hTERT protein, c-myc protein, NF-кB protein and survivin protein in 24 incised margin normal tissues, 21 adjacent-tumorous and 45 esophageal carcinoma specimens was examined by s-p immunohistochemical stain (IHC).2 The expression of hTERT protein in 8 incised margin normal tissues, 10 adjacent-tumorous and 27 esophageal carcinoma specimens was quantified by flow cytometry (FCM).3 The expression of hTERT mRNA in 24 incised margin normal tissues, 21 adjacent-tumorous and 45 esophageal carcinoma specimens was examined by in situ hybridization (ISH).Results1 Expression of hTERT protein and mRNA in various lesions of esophageal epithelialThe expressions of hTERT mRNA and protein in 45 esophageal carcinoma specimens, 21 adjacent-tumorous esophageal tissues and 24 incised margin normal tissues were examined by ISH and IHC respectively. The results showed that in both mRNA and protein level,the expression of hTERT in esophageal carcinoma (ISH:84.4%, IHC:88.9%) was significa- ntly higher than that in adjacent-tumorous esophageal tissues (ISH:38.1%, IHC:57.1%)and incised margin normal tissues (ISH:4.2%, IHC:12.5%) (P<0.05). There was a significant difference between 27 esophageal carcinoma specimens, 10 adjacent-tumorous esophageal tissues and 8 incised margin normal tissues quantitatively examined by FCM (P<0.05), FI value of hTERT in esophageal carcinoma group, adjacent-tumorous esophageal tissues group and normal mucosa group was 1.59±0.14,1.25±0.17,1.00±0.19 respectively.Results of ISH,IHC and FCM showed that the expression of hTERT wasn't correlated with age, sex , tumor differentiation or lymph node metastasis (P>0.05), but the high expression of hTERT in cancer tissues was significantly correlated with fibromembranous infiltration (P<0.01).There was a close correlation between the expressions of hTERT protein and hTERT mRNA in the esophageal carcinoma tissues (χ2=8.4586,P<0.01,c=0.3978).2 Expressions of c-myc in various lesions of esophageal epithelialThe expressions of c-myc in 45 esophageal carcinoma specimens, 21 adjacent-tumorous esophageal tissues and 24 incised margin normal tissues were exmined by IHC. The result showed that the expression of c-myc in esophageal carcinoma (75.6%) was significantly higher than that in adjacent-tumorous esophageal tissues (52.4%)and incised margin normal tissues (16.7%) (P<0.05).Results of IHC showed that the expression of c-myc wasn't correlated with age, sex or tumor differentiation (P>0.05), but the high expression of c-myc in cancer tissues was significantly correlated with fibromembranous infiltration and lymph node metastasis(P<0.05).3 Expressions of survivin in various lesions of esophageal epithelialThe expressions of survivin in 45 esophageal carcinoma specimens, 21 adjacent-tumorous esophageal tissues and 24 incised margin normal tissues were exmined by IHC. The result showed that the expression of survivin in esophageal carcinoma (82.2%) was significantly higher than that in adjacent-tumorous esophageal tissues (61.9%)and incised margin normal tissues (8.3%) (P<0.05).Results of IHC showed that the expression of survivin wasn't correlated with age, sex or tumor differentiation (P>0.05), but the high expression of survivin in cancer tissues was significantly correlated with fibromembranous infiltration and lymph node metastasis(P<0.05).4 Expressions of NF-кB in various lesions of esophageal epithelialThe expressions of NF-кB in 45 esophageal carcinoma specimens, 21 adjacent-tumorous esophageal tissues and 24 incised margin normal tissues were exmined by IHC. The result showed that the expression of NF-кB in esophageal carcinoma (80.0%) was significantly higher than that in adjacent-tumorous esophageal tissues (57.1%)and incised margin normal tissues (20.8%) (P<0.05).Results of IHC showed that the expression of NF-кB wasn't correlated with age, sex or tumor differentiation (P>0.05), but the high expression of NF-кB in cancer tissues was significantly correlated with fibromembranous infiltration and lymph node metastasis(P<0.05).5 The relationship between hTERT mRNA and hTERT proteinThere was a close correlation between the expression of hTERT mRNA and hTERT protein in the esophageal carcinoma tissues (χ2=8.4586, P<0.01, c=0.3978). 6 The relationship between hTERT protein and NF-кB,survivin,c-myc proteinThere was a close correlation between the expression of hTERT protein and NF-кB protein (χ2=5.6250, P<0.05, c=0.3333),survivin protein (χ2=6.8602,P<0.05, c=0.3646),c-myc protein (χ2=17.3864, P<0.05, c=0.5279) protein in the esophageal carcinoma tissues.7 The relationship between NF-кB protein and survivin,c-myc proteinThere was a close correlation between the expression of NF-кB protein and survivin protein(χ2=10.9840, P<0.05, c=0.4642),c-myc protein(χ2=5.8957, P<0.05, c=0.3404) in the esophageal carcinoma tissues.8 The relationship between survivin protein and c-myc proteinThere was a close correlation between the expression of survivin and c-myc protein in the esophageal carcinoma tissues (χ2=7.6294, P<0.05, c=0.3807).Conclusions1 The expression of hTERT in esophageal squamous cell carcinoma was significantly higher than that in adjacent-tumorous esophageal tissues and normal esophageal tissues, which indicated that hTERT could be correlated with carcinogenesis progression. In esophageal squamous cell carcinoma, the expression of hTERT with fibromembranous infiltration was significantly higher than that without fibromembranous infiltration. It indicated that the high expression of hTERT could be correlated with invasion of esophageal squamous cell carcinoma. There was no significant correlation between the expression of hTERT and tumor differentiation and lymph node metastasis. The close correlation between the expression of hTERT protein and hTERT mRNA in the esophageal carcinoma tissues indicated that the expression of hTERT protein indirectly reflected the expression of hTERT mRNA and the high expression of hTERT protein could be the result of high transcription of hTERT gene.2 The expression of c-myc in esophageal squamous cell carcinoma was significantly higher than that in adjacent-tumorous esophageal tissues and normal esophageal tissues, which indicated that c-myc could be correlated with carcinogenesis progression. In esophageal squamous cell carcinoma,the expression of c-myc with lymph node metastasis was significantly higher than that without lymph node metastasis and the expression of c-myc with fibromembranous infiltration was significantly higher than that without fibromembranous infiltration. It indicated that the high expression of c-myc could be correlated with invasion and metastasis of esophageal squamous cell carcinoma. There was no significant correlation between expression of c-myc and tumor differentiation.3 The expression of survivin in esophageal squamous cell carcinoma was significantly higher than that in adjacent-tumorous esophageal tissues and normal esophageal tissues, which indicated that survivin could be correlated with carcinogenesis progression. In esophageal squamous cell carcinoma, the expression of survivin with lymph node metastasis was significantly higher than that without lymph node metastasis and the expression of survivin with fibromembranous infiltration was significantly higher than that without fibromembranous infiltration. It indicated that the high expression of survivin could be correlated with invasion and metastasis of esophageal squamous cell carcinoma. There was no significant correlation between expression of survivin and tumor differentiation.4 The expression of NF-кB in esophageal squamous cell carcinoma was significantly higher than that in adjacent-tumorous esophageal tissues and normal esophageal tissues, which indicated that NF-кB could be correlated with carcinogenesis progression. In esophageal squamous cell carcinoma,the expression of NF-кB with fibromembranous infiltration was significantly higher than that without fibromembranous infiltration. It indicated that the high expression of NF-кB could be correlated with invasion of esophageal squamous cell carcinoma. There was no significant correlation between the expression of NF-кB and tumor differentiation and lymph node metastasis.5 There was a positive correlation between the expression of hTERT and NF-кB,survivin,c-myc protein in the esophageal carcinoma tissues, it indicated that there could be a positive cooperation between hTERT and NF-кB,survivin,c-myc in the carcinogenesis and development of esophageal carcinoma.6 There was a positive correlation between the expression of NF-кB and survivin,c-myc protein in the esophageal carcinoma tissues,it indicated that there could be a positive cooperation between NF-кB and survivin,c-myc in the carcinogenesis and development of esophageal carcinoma.7 There was a positive correlation between the expression of survivin and c-myc protein in the esophageal carcinoma tissues, it indicated that there could be a positive cooperation between survivin and c-myc in the carcinogenesis and development of esophageal carcinoma.