Study On The Relativity Between ER Gene Polymorphism And Systemic Lupus Erythematosus

Objective To study the mechanism of estrogen receptors in SLE and find the newmethods by which SLE can be rapidly diagnosed, and the new indexes of checking SLEclinical turnover, two aspect contents have been particularly investigated: 1 the difference ofthe ER gene polymorphism between the SLE patients and the normal group/the maleand the female SLE patients; 2 the difference of the three main gene types to the c variouskinds of clinical indications in the mechanism of SLE binding the clinical and the laboratorydata.Methods 1 RT-PCR-RFLP method is used to detect the polymorphism of the SLEpatients (82 cases) and health adults (82 cases), consequently confirming the sitesusing the gene sequencing. The gene segment lies in the first intron of the ERa and thelength is 1.3Kb. The enzyme cutting products are separated by electrophoretic method throughgelose and ethidium bromide which concentration is 1.0％. The consequence is judged using gelformatter and statistical analysis. 2 Induct the clinical and the lab data of the in hospital's patients(40cases) according to the diagnostic criteria and analyze the difference between the threemain genotypes of ER using the Statistical software (SPSS 11.5).Results1 detecting consequence of estrogen receptors' alleleThe length of the estrogen receptors is 1.3Kb after gene amplification. After cuttingthrough the restriction enzymes: PvuⅡand XbaⅠ, the results take on three cases: onestrap (PP and XX), two straps (pp and xx) and three straps (Pp and Xx). One strapexpresses that there hasn't enzyme cutting site. Two straps express that there has enzyme cutting cites in the two alleles. Three straps express that there only has onesite in the two alleles. The length after cutting is 850bp and 450bp, 910bp and 390bp.2 The ER genotype distribution after enzyme cuttingThe distribution difference is no statistical significance (P＞0.05) in the two groups.The main genotypes are Pp,pp, Xx,xx and p,x are the most common alleles. Thedistribute frequency of the ER genotype fairly approximate in common male andfemale, and the most common genotype are ppxx, PpXx; the distributive difference issignificant in the patients of male and female. The frequency of PpXx in malepatients (66.6％) is significantly higher than those in female patients (21.1％) and thenormal male grope (16.7％). The difference has statistical significance (P＜0.05). Butthe frequency of ppxx is obviously lower in male patients and higher in femalepatients than that in the male patients. The distributive difference is not significant inthe female patients and normal female. In the male and female patients we haven'tfind the PpXX.3 The clinical and lab dataWe mainly analyze the indexes of the three main genotypes in the hospital patients(40 cases). The frequency of plasma membrane, lung and brain damage in the patientswith PpXx is higher than the others. The frequency of lung damage in the patientswith PpXx is notable higher than that with ppxx, and the difference have statisticalsignificance (P＜0.05). The incidences rate of alopecia, photesthesis, Raynaud's sign,Edema of lower extremity, lymph gland are higher in ppxx than those in the others,but the difference haven't statistical significance. Photesthesis is more induced by theppxx. The incidences rate of rashes, joints damage, blood sedimentation,hematological system damage and heart damage are higher in ppxx than those in theothers, but the difference haven't statistical significance.Conclusion 1 The distributive difference of ER genotype is significant in male and female. The taking along ratio of PpXx in male is obviously higher than those infemale patients and normal male, which hint the genotype correlation with thesensitivity of the male patients. The frequency of ppxx in the female patients ishigher than that in the male patients. The difference is no significance. 2 Clinical dataare different in the three main genotypes, which hint that there are correlationsbetween the genotypes and parts of clinical information.