| Objective To research the Prescription of Zhuyun-Ⅲon T lymphocyte subset,NK cell,Fas/FasL of Spontaneous Abortion patient.To observe the effect ofprescription Zhuyun-Ⅲon T lymphocyte subset,NK cell,Fas/FasL ofSpontaneous Abortion rats by set up the animal model of renal-deficiency andluteal insufficiency abortion rats, investigate immunology nosogenesis ofSpontaneous Abortion and tocolysis mechanism of Zhuyun-Ⅲ.MethodClinical Research:According to internalize criteria, experiment groupconcluded 30 patients who were diagnosed as spontaneous abortion of both kidneyand spleen deficiency in the first trimester. Control group concluded 15 normalwomen in early pregnancy who requested artificial abortion. To dicover themechanism of action of Zhuyun-Ⅲon spontaneous abortion of both kidney andspleen deficiency, the content of CD3~+,CD4~+,CD8~+,CD4~+/CD8~+,NK cell in bloodof 15 normal pregnancy women were not only detected but also of 30 spontaneoussuffers by before and after two weeks of therapy with Zhuyun-Ⅲ.Experimental Research: Setting up the animal model of renal-deficiency andluteal-insufficiency abortion rats with Hydrea and Mifepristone and groupingthem at random in 4 teams: model with Zhuyun-Ⅲ,model with flavolutan,modelwithout herbs,controlled group.Comparing the content of CD3~+,CD4~+,CD8~+,CD4~+/CD8~+ and expression of Fas/FasLin the rats' blood and counting the rate of abortion, to investigate themechanism of spontaneous abortion and the immunology mechanism of action ofPrescription on Zhuyun-Ⅲ.ResultClinic study: Comparing with before and of after therapy withPrescription on Zhuyun-Ⅲof experiment group, variation of CD3+ is notsignificantly (P＞0.05). Comparing to control group heighten significantly (P＜0.01). Comparing to control group degrade significantly (P＜0.05).Comparing to control group odds ratio of odds ratio heighten significantly(P＜0.01). Comparing to control group heighten significantly (P＜0.05).Comparing to control groupβ-hCG of quantitative experiment group in blooddegrade significantly (P＜0.01). With Prescription Zhuyun-Ⅲtwo weeks later,there were 29 effective cases in the control group and 1 case which happenedSpontaneous Abortion again with total effective rate 96.7%.Comparing withbefore and of after therapy with Prescription on Zhuyun-Ⅲof experiment groupratio of CD3~+ didn't (P＞0.05).After prescription Zhuyun-Ⅲratio of CD8~+heighten significantly (P＜0.01), while ratio of CD4~+ and CD4~+/CD8~+ and percentcontent of NK cells degrade significantly (P＜0.01).Experimental study:Comparing with other 3 groups CD3~+ is the highest incontrolled group which isn't significant (P＜0.05), while CD4~+ and CD4~+/CD8~+is the highest in controlled group which is significant(P＜0.01)and CD8~+ don' tdegrade significantly(P＞0.05).Comparing to controlled group CD4~+ and CD4~+/CD8~+degrade significantly in the model with Zhuyun-Ⅲand flavolutan (P＜0.01).Comparing with model without herbs and model with Zhuyun-Ⅲand flavolutanCD4~+ and CD4~+/CD8~+ area' t significantly (P＞0.05).Comparing to model withoutherbs index of average dyeing intensity of expression of index FasL degradesignificantly and Fas heighten significantly in the controlled group(P＜0.05). Comparing to controlled group index ofexpression of FasL heightensignificantly and Fas degrade significantly in the model with flavolutan(P＜0.05). Comparing to model without herbs index of dyeing intensity indexof expression of FasL don' t change significantly in the model with Zhuyun-Ⅲand flavolutan (P＞0.05).Conclusion1. It was conformed that content of CD4~+ and ratio of CD4~+/CD8~+ raiseobviously,with decreased content of CD8~+ and raised percentage of NK cellsspontaneous abortion in the first trimester.The research also demonstratedthat imbalance of proportional T lymphocyte subset and raised percentage ofNK cells might be one reason of spontaneous abortion.2.1t was disclosed that Comparing to normal rats in early pregnancythere has been not marked change for CD3~+, while content of CD4~+ and ratio ofCD4~+/CD8~+ raise obviously and index of positive staining of expression of FasLheighten significantly in renal-deficiency and luteal-insufficiency abortion rats. It also provide that proportion disturbance of lymphocyte subset andexcess of immunologic function land raised content of CD4~+ and ratio of CD4~+/CD8~+ lead to spontaneous abortion in the first trimester.3. The research indicated that Prescription Zhuyun-Ⅲcan invigorate thekidney and spleen, so that it can retrieved the proportion disorder oflymphocyte subset by decreasing the content of CD4~+ and ratio of CD4~+/CD8~+tomake CD4~+ and CD8~+ T lymphocyte educe immune coordination in immune responseof body.It also provided the scientific evidence that Prescription Zhuyun-Ⅲcan heighten expression of FasL of decidua tissue in rats which werespontaneous abortion in the first trimester to make Fas lymphocyte apoptosispromptly so that protect nutrient cell from aggression of lymphocyte andcontinue gestation. |
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