The Expressions Of BFGF And FGFR Anfter Cerebral Ischemia Reperfusion And The Protective Effects Of Phycocyanin In Rats

【Objective】To study the effects of the expression of SOD, nNOS, eNOS and iNOS after focalcerebral ischemia reperfusion in rats and the protection of phycocyanin on the neurons.【Methods】A rat middle cerebral artery occlusion reperfusion (MCAO) model was producedusing the intraluminal filament method. The rats were divided into three groups: sham operation group(n=4), model control group (n=24) and phycocyanin group (n=24). After MCAO, the neurobehavioraltesting of all rats was made. The expression of nNOS, eNOS, iNOS and SOD were determined byimmunohistochemical staining and the protection of phycocyanin on the neurons.【Results】(1) In the sham operation group, there is only a few nNOS-positive cells were seen inthe normal cerebral tissue. In the model control group, the upregulation of nNOS began 6h afterischemia, reached a maximum at 24h, then subsided gradually, but still in high level. In thephycocyanin group, nNOS-positive cells were also mainly in cortex and striatum, but the number ofthe cells were significantly less than the number of the model control group. The time-phase pattern ofnNOS is similar to the pattern of the model control group.(2) In the sham operation group, there is noiNOS-positive cells were seen in the normal cerebral tissue. In the model control group, there is noiNOS-positive cells began 6h after ischemia, the upregulation of iNOS began 24h, then reached amaximum at 72h, then subsided gradually,. In the phycocyanin group, were also mainly in cortex andstriatum, but the number of the cells were significantly less than the number of the model controlgroup. The time-phase pattern of iNOS is similar to the pattern of the model control group. (3) In thesham operation group and the model control group, there is only a few eNOS-positive cells were seenin the normal cerebral tissue. In the model control group, the upregulation of eNOS began 6h afterischemia, reached a maximum at 24h and subsided at 3d, but still in high level. In the phycocyaningroup, eNOS-positive cells were also mainly in cortex and striatum, but the number of the cells weresignificantly more than the number of the model control group. The time-phase pattern of eNOS issimilar to the pattern of the model control group. (4) In the sham operation group and the modelcontrol group, there is a few SOD-positive cells were seen in the normal cerebral tissue. In the modelcontrol group, the upregulation of SOD began 6h after ischemia, reached a maximum at 24h andsubsided at 3d, but still in high level. In the phycocyanin group, SOD-positive cells were also mainlyin cortex and striatum, but the number of the cells were significantly more than the number of themodel control group. The time-phase pattern of SOD is similar to the pattern of the model controlgroup.【Conclusions】The over-expression of nNOS and iNOS in the model control group weresignificantly more than the number of the sham operation group in ischemic brain injury after MCAO.It infer that over-expression of NOS can product over-expression of NO, that can the protection of onthe neurons and injuration in the brain Phycocyanin can inhibit the over-expression of nNOS and iNOS in the cerebral cortex, and improve eNOS might play an important role in the protection ofischemic neurons. Phycocyanin can improve SOD significantly and clean up free radical and protectischemic neuron after focal cerebral ischemia/reperfusion in rats.