Expression Of Aquaporin-1 MRNA In Human Lens Epithelial Cell

PurposeAQP1 is plentiful in epithelial cells of liquid absorption and secretion and in endothelial cells which may cooperate with water transcellular transport. AQP1 distribution in lens subcapsule epithelial cell has been documented. Changes in expression levels and function directly related to the the abnormality of lens metabolism . Semi-quantitative RT-PCR and computer-imaging analysis system were used to detect the expression of AQP1mRNA in lens subcapsule epithelial cells and compare the difference of AQP-1mRNA expression between clear lens and age-related cataract lens epithelial cell and the difference of AQP-1mRNA expression between HLE-B3 and age-related cataract lens epithelial cell. It is hoped that we can provide some clues for the illuminate of age-related cataract.Materials and methodsSemi-quantitative RT-PCR1,Sample:9 cases of anterior lens capsules of clear lens and 31 cases of anterior lens capsules of age-related cataract were collected (14cases of cortical cataract and 17cases of nuclear cataract). These samples were stored at -70-70℃.Human lens epithelial cell line is HLE-B3.2,Methods: Total RNA extraction, Reverse transcription reaction, Polymerase chain reaction. The electrophoresis of 5μl PCR products was performed at 45mV for 40 min in 2% agarose gel. And the bands were viewed and photographed under UV illumination, the data was preserved in 10Kodak computer-imaging analysis system.3,Statistical analysis: Statistics analysis was performed using SPSS 12.0 computer software.ResultThere was statistically significant difference in the AQP1mRNA expression between clear and cortical cataractous lens epithelial cells (P< 0.01). There was statistically significant difference in theAQP1mRNA expression between clear and nuclear cataractous lens epithelial cells (P<0.01). There was no statistically significant difference in the AQP1mRNA expression between cortical and nuclear cataractous lens epithelial cells (P>0.05). There was statistically significant difference in the AQP1mRNA expression between HLE-B3 cell line and cortical cataractous lens epithelial cells (P< 0.01). There was statistically significant difference in the AQP1mRNA expression between HLE-B3 cell line and nuclear cataractous lens epithelial cells (P< 0.01).ConclusionThe expression of AQP1mRNA in cortical cataractous lens epithelial cells was lower than in clear lens epithelial cells. The expression of AQP1mRNA in nuclear cataractous lens epithelial cells was lower than in clear lens epithelial cells. There was no significant difference in the AQP1mRNA expression between cortical and nuclear cataractous lens epithelial cells. The expression of AQP1mRNA in cortical cataractous lens epithelial cells was lower than in HLE-B3 cell line. The expression of AQP1mRNA in nuclear cataractous lens epithelial cells was lower than in HLE-B3 cell line. AQP1 may play a physiological role in the occurrence and development of age-related cataract.