The Expression And Significance Of Early Growth Response-1 In Selenite-induced Cataract As Well As In Lens Epithelia Cells Which Are Impaired By H₂O₂

Objective To explore the expression and significance of early growth response-1(Egr-1) in different stage of selenite-induced cataract and in lens epithelial cells which are impaired by H₂O₂.Methods 1.45 Sprague-Dawley rat pups was divided into control group,normal group and experiment group. The experiment group was subcutaneous injected of 3.46mg sodium selenite/kg body weight, while the control group was subcutaneous injected of corresponding sodium chloride, nothing was injected to the normal group. After injecting sodium to Sprague-Dawley rats,the degree of lens opacity was observed by slit-lamp. On d1,3,7, the Egr-1 expression was detected by immunohistorychemistry(SP assay) respectively.2. The epithelial cells was cultured in vitro,after treated by H₂O₂, the Egr-1 expression was detected by immunohistorychemistry(SP assay).Results 1. In the experiment group,the lens opacity continuously aggravated, dust opacity and crico-opacity were formed d 3 after injecting and typical nuclear cataract was formed at d 7 after injecting. While in the control and normal group,the lens were keeping transparent2. The Egr-1 expression was weakly positive at d 1 after injecting,positive or hadro-positive at d 3 after injecting, while weakly-positive at d 7 after injecting .While in the control and normal group, and Egr-1 expression was negative.3. 2h after treated with H₂O₂ ,the expression of Egr-1 in the epithelial cells was hadro-positive ;the opatical density value was 0.55±0.08;8h after treated withH₂O₂, the expression of Egr-1 in the epithelial cells was positive ;the opatical density value was 0.40±0.09; The difference between the two groups was significant ,while 24h after treated with H₂O₂ and in control group the expression of Egr-1 in the lens epithelial cells was negative.Conclusion Egr-1 was expressed at early stage of selenite-induced cataract, and it also expressed in the early stage of the epithelial cells afer treated with H₂O₂, it may be one of the early effective factors in the development of oxidative damaged cataract.