| Purpose:1. Cataract model in rats, and sodium selenite cataract model induced by the mechanism of lens opacity.2. To explore Qu Zhang Point cryosurgery and changing the freezing point sites on the selenite cataract model induced by inhibition of the progress of lens opacity and delay mechanisms.3. By Qu Zhang Point cryosurgery and changing the freezing point cryotherapy sites the way comparative analysis of the latter effect for the treatment of senile cataract the predictability and feasibility.Methods:35 rats were slit lamp examination to exclude the lens disease. 5 rats were randomly selected as normal control group, the remaining rats were randomly divided into 3 groups of 10 each, every other day subcutaneous injection in the neck, 1mmol / L of sodium selenite were injected three times the normal group was given 0.9% saline, monitoring changes in lens opacity. Lens opacity appears to be just when the treatment group of Qu Zhang Point cryosurgery to cured by Qu Zhang Point cryosurgery, 1 time a week, 5 Mon treatments, methods, invented by Mr Li You cai'Qu Zhang Point cryosurgery. Freezing changes the treatment sites of the rats in the rat limbal 12 randomly selected 4-bit each time point to cryosurgery, use the same methods as Qu Zhang Point cryosurgery group. Through the slit lamp to observe and record the progress of rat lens opacification. Lens opacity classification criterion used by the laboratory method of Ophthalmology, University of Oxford. 6wk rats were sacrificed, remove the lens to determine which water-soluble protein, glutathione (GSH), glutathione reductase (GR) and catalase (CAT) activity.Results:1. In addition to the normal group than the other three rats eyes were cloudy began in 1wk, 2wk～6wk untreated cataract lens opacity group eyes rapid progress, while the two treatment group eyes the progress of lens opacities compared with the relative B group slow, and the turbidity and light in the B group, the difference was significant (right:P=0.000,.left:P=0.002).2. Lens soluble protein content, compared with the normal group, no treatment group were significantly lower, with statistical significance (right: P = 0.010, left: P = 0.001), Qu Zhang Point cryosurgery right eye point freezing group, frozen sites change the group with normal control eyes, but no statistical significance (P>0.05), the left eye of Qu Zhang Point cryosurgery group were significantly lower than normal (P = 0.016). 3.GR activity, each rat eye lens compare the difference was not statistically significant (P>0.05);4.GSH comparison, compared with normal rats of cataract eyes without treatment, Qu Zhang Point cryosurgery group and removing of point freezing treatment group,eyes sites to change the lens GSH concentration decreased and there was significant difference (P = 0.000, P = 0.000, P = 0.000).5.CAT activity, compared with normal rats of cataract eyes without treatment, Qu Zhang Point cryosurgery group and removing of point freezing treatment group sites change CAT activity was significantly lower lens eyes, there was significant difference (P = 0.000, P = 0.000, P = 0.000). Qu Zhang Point cryosurgery group and removing of point freezing treatment group sites change CAT activity in the treatment group than in untreated patients with cataract, right eye were 41.4% and 35.9% (P = 0.003, P = 0.012), left eye were 32.3% and 27.4% ( P = 0.008, P = 0.031), with statistical significance.6. Pigmentation, Qu Zhang Point cryosurgery group and removing of point freezing treatment group change in position on the rats in the treatment group were frozen treat limbal formation of cold spots are an instant, then cold spots disappeared, 6wk few Qu Zhang Point cryosurgery group limbal frozen rats frozen sites pigmentation appears at different levels, and this reflected in the frozen site failed to change the treatment group there.Conclusion:1. Selenite cataract model induced cataract pathogenesis and treatment evaluation of reliableModel.2. The lens oxidative damage may run through the development of cataracts.3. Qu Zhang Point cryosurgery group onset of cataract in rat lens opacity has good inhibition, delay Slow effect.4. Qu Zhang Point cryosurgery group and removing of point freezing of randomly selecting for each 4 sites treatment group Limbal rats were in dialogue, impaired inhibition of rat lens opacity and delay comparison of the role of no significant difference, while the later can be better distributed by the freezing of the local tissue irritation and damage caused.5. According to dispel the Qu Zhang Point cryosurgery treatment of senile cataract is not mature research results standardized order (State Administration of Traditional treatment technology issues, the National Bureau 2001ZL27 number of Chinese medicine), is expected to limbal frozen each 4 sites were randomly selected treatment age-related cataract will get better effect. |
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