| OBJECTIVE To investigate the effects of oxidized low density lipoprotein(ox-LDL) and/or high glucose on expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and interleukin-1β(IL-1β) in human umbilical vein endothelial cell line (HUVEC-12), and the inhibitory effect of PPARγagonist on ox-LDL and/or high glucose- induced expression of LOX-1.METHODS Human umbilical vein endothelial cells-12 were treated with ox-LDL at different concentrations (0,20,40,80μg/ml) and glucose (0,5.6, 11.2, 22.4, 44.8mmol/L) for 24h, respectively. LOX-1 mRNA expression levels were determined by reverse transcription-polymerase chain reaction (RT-PCR),and the protein expression levels were evaluated by Western blot and flow cytometry, respectively. Interleukin-1βmRNA and protein levels were determined by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells-12 were co-incubated with ox-LDL (40μg/ml), high glucose(22.4 mmol/L), or combination of oxLDL(40μg/ml) and high glucose (22.4 mmol/L) for 24h. Furthermore, the cells as described above were treated with PPARγagonist ciglitazone (10μmol/L) to illustrate the inhibitory effect of PPARγagonist on the induced expression of LOX-1 by ox-LDL or high glucose.RESULTS The results showed that ox-LDL (20, 40, 80μg/ml) and high glucose (22.4, 44.8 mmol/L) increased LOX-1and IL-1βexpression in both protein and mRNA levels in a dose-dependent manner, respectively. LOX-1 expression was also up-regulated by IL-1βas showed in both protein and mRNA levels. PPARγagonist ciglitazone inhibited the upregulation of LOX-1 expression induced by ox-LDL or high glucose as in both protein and mRNA levels. CONCLUSION Ox-LDL and high glucose up-regulate the expression of LOX-1 and IL-1βin a concentration-dependent manner. IL-1βmay up-regulate LOX-1 expression. PPARγagonist ciglitazone may decrease LOX-1 expression level induced by ox-LDL or high glucose.
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