| Obejective: To investigate the effect of donor-derived bone marrow mesenchymal stem cells(BM-MSC) on hematopoietic reconstitution and acute graft-versus-host disease(aGVHD) after allogeneic bone marrow transplantation(Allo-BMT). Method: We used female Wistar rats as recipients and used male SD rats as donors. All wistar rats were randomized to A,B,C and D groups which all received total body irradiation(TBI) 8.5Gy 4-6 hours prior to transplantation. Group A received equivalent dose of RPMI 1640; Group B received donor bone marrow cells (2×10~8/kg) via tail vein, group C received donor bone marrow cells (2×10~8/kg)+ spleen cells(3×10~8/kg); group D received donor bone marrow cells (2×10~8/kg)+ spleen cells (3×10~8/kg) + donor- derived BM-MSC(1×10~7个/kg) which were isolated and cultured to the fifth passage(P5) in vitro. Afterword, survival time, leucocyte counts of peripheral blood, .aGVHD manifestation of clinical and pathologic were evaluated and Y chromosome was detected from recipients. Result: Group A rats died within 15 days and showed hematopoietic failure with bone marrow pathologic evidence. Leucocyte counts of peripheral blood increased more significantly in group D than group B and C at the end of 2 weeks after transplantation (P<0.05). 8 rats survived more than 50 days in group B, rats of group C and D died within 50 days, the died rats showed aGVHD manifestation with clinical and pathological evidence, but cotransplantation of BM-MSC could delay the onset time of aGVHD, decrease clinical score and prolong the survival time in group D compared with group C(P<0.05),bone marrow cells of recipients showed Y chromosome derived from donors. Conclusion: The TBI regime of pretreatment is feasible. Only allogenic bone marrow cells inftusion can not induce typical aGVHD, cotransplantation of bone marrow cells and spleen cells can induce typical aGVHD. BM-MSC can improve hematopoietic reconstitution and alleviate aGVHD after Allo-BMT. |
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