Identification Of A Novel Subset Of CD19～+ PDCA-1～+ Mouse B Lymphocytes

B lymphocytes are a kind of powerful immunocytes which play important roles in both humoral immunity and cell immunity. Here we found a novel subset of CD19+ PDCA-1+ mouse B lymphocytes in the model of inflammation by injection with LPS or CpG-ODN and then identify the phenotype and cytokine profile of them. Our data indicates that CD19+ PDCA-1+ cells widely occurs in the spleen, the bone marrow, and mesenteric lymph nodes in the models of inflammation induced by LPS or CpG-ODN, which suggests the generation of CD19+ PDCA-1+ cells is related with TLR signals. Additionally, the novel subset express CD19,PDCA-1,F4/80,B220,mIgM,mIgD,CD45RB,CD44,CD23 and cytokines of Th1 type. Finally, we demonstrated the critical role of IFN-γ, working with TLR signals, in the generation of CD19+ PDCA-1+ cells. In conclusion, we have successfully identified a new subset of B cells which may be involved in the process of inflammation or immune regulation, which needs to be investigated in the future.