Study On Effects Of Protein Tyrosine Kinase Inhibitor Staurosporine In Gastric Carcinoma Cells

Objective To explore the effects of PTK inhibitor staurosporine (SP) on proliferation of SGC7901 in vitro, and discover the role of MAPK signal transduction pathway mediated by PTK to gastric cancer.Methods The changes of morphology of SGC7901 treated with SP were observed using inverted microscope;Western blot analysis was used to examine the changes of protein level of ERK1, ERK2, p-ERK in SGC7901 treated with SP in different concentration;MTT assay was employed to determine the growth inhibition of SP on SGC7901.Result Phosphorylation of ERK,which were downstream molecules of gastric cancer cell line SGC7901,was obviously decreased after being treated with SP ( P < 0.05 ), while no significant differences of non-phosphorylated ERK expressions under different SP concentrations were detected(P>0.05);Using MTT,it was clear that the growth of untreated SGC7901 went into platform phase on the fourth day,while the growth of SGC7901 has been inhibited by either SP concentrations since the first day,both SP at 40ng/ml and 80ng/ml nearly inhibit the growth of SGC7901 completely.Conclusions PTK inhibitor staurosporine (SP) obviously inhibited proliferation of SGC7901 in vitro,and decreased the expression of p-ERK in MAPK transduction pathway of SGC7901.