| A method was established to determine the concentration of taurocholic acid of liver, kidney, brain tissue and blood serum by solid phase extraction(SPE) with reversed-phase high performance liquid chromatography(RP-HPLC) in rats.To observe concentration changes of blood serum and distribution of tissue of taurocholic acid in rats . in the paper , Pharmacokinetics of Taurocholic Acid were studied.Taurocholic acid concentration in liver, kidney, brain tissue and blood serum was determined in rats by SPE with RP-HPLC, The results showed that taurocholic acid concentration in serum has linear relationship with peak area during 0.15625μg/ml～10.0μg/ml, regression equation was Y=40388X+42689, R2=0.9978 ; The lowest detectable limit of taurocholic acid was 0.02μg/ml.; The average recovery was 99.05%; The RSD of inner-day was 2.21%～4.29%; The RSD of intra-day was 3.30%～6.64%; Using taurocholic acid standard preparation, to get the Standard Curve and then calculated taurocholic acid concentration in tissue relatively. The results only reflected relative changes law of taurocholic acid concentration couldn't stand for the real taurocholic acid concentration in tissue. Stability of tissue sample was investigated accurately by experiments; the results were similar to blood sample results. The method was simple, accurate, sensitive, feasible and suitable for analyzing taurocholic acid concentration.Taurocholic acid (0.25g/kg·b·w) were intragastric administrated in single dose. The liver , kidney , brain tissue and blood serum level of taurocholic acid were determined at different time by SPE with RP-HPLC, The data were analyzed through DAS ver 1.0 pharmacokinetics software .The results were as followed: (1) The drug-time curve of blood serum was suitable to open two-compartment, Its pharmacokinetics formula was C= 6.899e-0.186t+0.386e-0.002t-7.285e-0.441t,The pharmacokinetics parameters were: Tmax 4.0h,Cmax 6.795mg/L,T1/2ka 1.573h,T1/2α3.724h,T1/2β396.097h,AUC 38.894mg/L·h,Vd/F 553.899L/kg,V1/F 34.314L/kg,CL/F 0.969L/h/kg . (2) The drug-time curve of liver was suitable to open two-compartment, Its pharmacokinetics formula was C=53.156e-0.116t + 48.254e-0.002t-101.41e-1.139t, The pharmacokinetics parameters were: Tmax 8.0h,Cmax 129.641mg/L,T1/2ka 0.608h,T1/2α5.971h,T1/2β283.053h,AUC 4524.148mg/L·h,Vd/F 5.063L/kg,V1/F 2.465L/kg,CL/F 0.012L/h/kg. (3) The drug-time curve of kidney was suitable to open one-compartment , Its pharmacokinetics formula was C= 4.412(e-0.001t-e-0.058t),The pharmacokinetics parameters were: Tmax 48.0h , Cmax 9.205mg/L,T1/2ka 11.859h,T1/2 623.095h,AUC 808.31mg/L·h,Vd/F 56.67L/kg,CL/F 0.063L/h/kg. (4) The drug-time curve of brain was suitable to open one-compartment tissue, Its pharmacokinetics formula was C= 0.941(e-0.006t-e-0.582t),The pharmacokinetics parameters were: Tmax 48.0h,Cmax 0.956mg/L,T1/2ka 1.192h,T1/2 114.539h,AUC 74.951mg/L·h,Vd/F 265.628L/kg,CL/F 1.607L/h/kg. from above, we can draw a conclusion that Taurocholic acid was absorbed quickly and distributed extensively and eliminated slowly after taurocholic acid was taken administration in rats. It's good to bring into full play the clinic pharmacokinetics functions. |
PDF Full Text Request