Effects Of Insulin Receptor On T-helper Lymphocyte Differentiation In Asthmatic Patients In PBLs In Vitro

Objective To investigate the effects of insulin receptor and insulin receptor antibody on T-helper lymphocyte differentiation in asthmatic patients in PBLs in vitro and it's potential mechanism.Methods Blood samples were obtained from asthmatic patients(n=11). Separated peripheral blood lymphocytes(PBLs)were placed in serum free medium.Each PBLs sample was divided into insulin receptor(α-subunit) antibody treated group,insulin receptor(β-subunit)antibody treated group and the control group.All samples were stimulated with PMA and Ionomycin for 6h at 37℃in the presence of brefeldin-A and Monensin.The percentage of IFN-γ~+cells and the percentage of IL-4~+ cells and Thl/Th2 ratio were determined by Flow cytometry.Results 1.As compared with the control group,the percentage of IFN-γ~+ cells and Th1/Th2 ratio in the insulin receptor(α-subunit)antibody treated group showed a significantly increase(P＜0.05),the percentage of IL-4~+ cells showed no significant differences between the two groups.2.The percentage of IFN-γ~+ cells and IL-4~+ cells were no statistically significant between the insulin receptor (β-subunit)antibody treated group and the control group,howerer,Th1/Th2 ratio showed a significantly increase in the insulin receptor(β-subunit)antibody treated group(P＜0.05).3.Compared with the insulin receptor(α-subunit) antibody treated group,the percentage of IFN-γ~+cells and IL-4~+ cells and Th1/Th2 ratio were no statistically significant in the insulin receptor(β-subunit) antibody treated group.Conclusion Insulin receptor modulates the differentiation and phenotypes of T-helper lymphocyte.Insulin receptor(α-subunit)antibody and insulin receptor (β-subunit)antibody promoted the precursor Th cells(ThO)differentiate to Thl and increased the percentage of Th1 cells.It skews the Th1/Th2 balance to Th1.