The Inhibited Effect Of Compound Traditional Chinese Medicine Ointment On Hypertrophic Scars Of Rabbit Ears

Objective:To investigate the effect and mechanism of Compound Traditional Chinese Medicine Unguent on the hypertrophic scar(HS)in rabbit ears.Methods:The experiment was completed in the Laboratory of Department of Plastic Surgery,Xijing Hospital of the Fourth Military Medical University from July to December in 2006.①16 New Zealand rabbits(3～4 months year old)were chosen as experiment animals.②The models of HS in ears were reproduced.All rabbits were classified into 4 groups randomly:the experiment group(treated by the compoud traditional Chinese medicine ointment),the medicineⅠcontrol group(treated by contractubex),the medicineⅡcontrol group(treated by asiaticoside)and the blank control group.There were eight ears(forty-eight wounds)in each group.When epithelized,the wounds were treated respectively by the compoud traditional Chinese medicine ointment,contractubex,and asiaticoside three times per day, except that the blank group was treated by nothing.③On the 30^thday and the 100^thday four specimens were taken from each rabbit.And then the appearance and pathology were observed;the scar thickness and Hypertrophic Index(HI), blood flow of micro-circulation,collagen,a-smooth muscle actin and transforming growth factor-betal were tested;fibroblasts number was count.④T test was used in the comparison of two groups;variance analysis was used in the comparison of multitude groups.Results:All the ears of 16 rabbits entered the result analysis.OThe quantity of HS in the experiment group is fewer than that in the medicineⅡgroup and the blank group,but more than the medicineⅠgroup.②Blood flow of micro-circulation was higher significantly in the experiment group than that in the control groups(P＜0.05).③Long-term curative effect of the compoud traditional Chinese medicine ointment is conspicuous.④Fibroblast counting on the 30^thday was obviously lower in the experiment group than that in the blank group(P＜0.05);on both of the 30^thand 100^thday,expression of a-smooth muscle actin was obviously lower in the experiment group than that in the blank group(P＜0.05).⑤Quantitative determination and semi-determinalion of collagen on the 30^thand 100^thboth were lower in the experiment group than that in the blank group(P＜0.05).6 Expression of transforming growth factor-betal on the 30th day was obviously lower in the experiment group than that in the blank group(P＜0.05).Conclusions:The compoud traditional Chinese medicine ointment can inhibit the formation of the hypertrophic scars of rabbit's ears,lessen the degree of fibrosis of hypertrophic scar,reduce the fibroblasts and inhibit the hypertrophic scar contracture.