NDRG2 Inhibits PDGF Production In Macrophage-derived Foam Cell And VSMC Proliferation

Objective: The aim of this study was to investigate the effect of NDRG2 on PDGF expression and its mechanism in the macrophage-derived foam cells, further explored its possible effects on the proliferation rate of vascular smooth muscle cells. Methods: RAW264.7 was transfected transiently with the NDRG2, then was stimulated by oxLDL, the expression of PDGF was detected in the macrophage-derived foam cell. RAW264.7 was transfected transiently with the NDRG2, then was stimulated by LPC, the expression of PDGF was detected in the macrophage. RAW264.7 was transfected transiently with the NDRG2 SiRNA, then was stimulated by LPC, the expression of PDGF was detected in the macrophage. RAW264.7 was transfected transiently with NDRG2 SiRNA and incubated with respective inhibitor of ERK, P38 and c- JNK, the secret expression of PDGF in RAW264.7 was detected. that the RAW264.7 cell was transfected with NDRG2 and NDRG2 SiRNA transiently and was stimulated by LPC, the cell culture supernatant was collected. VSMC was incubated with the cell culture supernatant. Then VSMC proliferation rate was detected. Results: Compared with the control cell, NDRG2 can inhibit PDGF expression in the macrophage-derived foam cell significantly. Compared with the control RAW264.7, NDRG2 can inhibit PDGF expression that was induced by LPC in macrophage significantly.Compared with the control RAW264.7, interfering NDRG2 expression can promote PDGF expression in the macrophage and also promote PDGF expression was induced by LPC in macrophage significantly. After ERK expression was inhibited, the secret expression of PDGF in macrophage with interfering NDRG2 expression was decreased significantly. Compared with that VSMCs were incubated with the cells culture supernatant of RAW264.7 with NDRG2 normal expression, VSMCs proliferation was suppressed significantly by incubating with the cell culture supernatant of RAW264.7 that were transfected transiently with NDRG2 and stimulated by LPC. Conversely, VSMC proliferation rate was promoted significantly by incubating with the cells culture supernatant of RAW264.7 that was transfected transiently with NDRG2 SiRNA and stimulated by LPC. Conclusion: NDRG2 can inhibit PDGF expression in macrophage-derived foam cells, and further inhibited the around VSMC proliferation. It involves ERK/MAPK signal transduction pathway during NDRG2 inhibit PDGF expression.