| Objective: The purpose of this study is to investigate the methods of separation,culture and identification of human mesenchymal stem cells(hMSCs), to observe the osteogenesis characteristics of hMSCs under bone induction conditionand to investigate the feasibility as the seed cells of periodontal tissue engineering . After combing hMSCs with Bio-gide collagne membrane , the biocompatibility of this complex were evaluated.Method: HMSCs are separated and cultured in vitro. To observe the shape, to determine the generation,and to detect the cell surface antigen. The favourable tert-hMSCs are cultured under different culture condition(essay group: osteogenesis induction culture solution, control group: common culture solution).The alkaline phosphatase (ALP)level and the ability to produce mineralized nodules were examined. HMSCs were cultured combined with Bio-gide collagen membrane in vitro.,and the morphological characters,cell attachment,and proliferation were detected by inverted phase contrast microscope and scanning electron microscope.Result: hMSCs could be effectively isolated and purified by density gradient centrifugation and adherence to the culture plastic.By bone induction hMSCs have higher levels of ALP(p〈0.05) and can produce minel nodules in vitro about 28 days later. HMSCs could be attached to and extend on Bio-gide collagen membrane and proliferated in amulti-layer fashion.Conclusion: It is safe and convenient to make use of hMSCs; hMSCs may be induced into osteoblast; hMSCs can provide ideal seeding cells for construction of tissue engineering alveolar bone . Bio-gide collagen membrane have good biocompatibility with hMSCs and potential scaffolds for periodontal tissue engineering.
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