Measurement And Meaning Of Insulin-like Growth Factor-I In Serum And Follicular Fluid During Controlled Ovarian Hyperstimulation Cycle

Objective To observe the relationships among the levels of insulin-like growth factor-I (IGF-I) in serum before and after stimulated and follicular fluid during controlled ovarian hyperstimulation cycle in vitro fertilization and embryo transfer(IVF-ET); The relationships among IGF-I and gonadal hormone and gonadotropin in follicular fluid, and assessing the impacts of IGF-I on parameters of IVF-ET.Method 42 cycles of IVF-ET patients were used the same superovulation regimen. IGF-I concentration in serum and follicular fluid samples were measured by using emzyme-linked immunosorbent assay, gonadal hormone[estradiol(E2),progesterone (P)],gonadotropin [luteinizing hormone(LH), follicle-stimulating hormone(FSH)] in follicular fluid samples were measured using chemiluminescence analysis. The follicles were divided into three groups: low reactive group, moderate reactive group, and high reactive group according to the number of follicle that diameter≥14mm on OPU day. Register the numbers of clinical pregnancy and the correlated parameters (number of obtained eggs, number of oosperm, fertility rate, number of cleavage, cleavage rate) for the further contrast and analysis.Results 1. There were no significant changes among the levels of IGF-I in serum before and after stimulated and follicular fluid in low reactive group(p>0.05), as well as in the moderate reactive group, and in high reactive group. There were no significant changes among the levels of IGF-I in serum before stimulated in three groups(p>0.05), same as in serum after stimulated and in follicular fluid. 2. In pregnancy group and non-pregnancy group, the difference of IGF-I in follicular fluid was non-significant(p>0.05). The difference of E2 was significant (p<0.05). The difference of P was non-significant (p>0.05). The difference of LH was significant (p<0.05). The difference of FSH content was non-significant(p>0.05).3. Follicular fluid IGF-I level was positively correlated with E2(r=0.38,p <0.05), it was nondistinctive correlation with P(r =0.32,p>0.05), and was positively correlated with LH (r=0.45,p<0.05),while it was negatively correlated with FSH (r =-0.31, p <0.05).4. In follicular fluid, IGF-I was nondistinctive correlation with number of obtained egg(r=0.25,p>0.05), number of oosperm (r=0.36,p>0.05) and number of cleavage(r=0.31,p>0.05). It was positively correlated with rate of oosperm (r=0.31, p>0.05) and rate of cleavage (r=0.42, p<0.05).Conclusion IGF-I exists in follicular fluid, which could come from peripheral blood circulation and may be worked in adjusting the process of follicle growth. IGF-I contributed the synthesis and secretion of E2 and LH, and at the same time it could coordinate the gonadotropin secretion. It is not significantly effect on pregnancy, while has certain relationship with the development of fertilized eggs.