Effect Of Cordycepin On Inhibiting Delayed Type Hypersensitivity

Objective: To observe the effect of cordycepin on inhibiting delayed type hypersensitivity and to investigate the mechanism .Methods: The mouse model of delayed type hypersensitivity was established by sensitizing the skin of Kunming mice with 2,4- dinitrochlorobenzen (2,4-DNCB). There are two experimental groups respectively treated with different dosages of cordycepin: 12mg/kg and 16mg/kg and one negative control group treated with normal saline. All the mice were intraperitoneally injected at 24h interval (the last interval was 4 hours). After eight days, all the mice were sacrificed. And then we calculated the differences of the thickness and the weight between the left and right earlaps of every mouse and the edema rate, then used statistical methods to compare the edema rate and the spleen index among all the groups. After HE staining, the pathological changes of inflammatory earlap and spleen were observed and compared among these three groups.Results: Cordycepin alleviated the erythema, edema and the infiltration of peripheral mononuclear cells in the earlaps, so lowering the differences of the thickness and weight between left and right earlaps in the experimental groups. But it showed no significant effects on the spleen index and the spleen pathological changes. Compared with that in the negative control group, the anti-inflammatory effects of cordycepin in two experimental groups showed significant differences (P<0.05) and were correlated with dosage. Even between two experimental groups, the result was the same (P<0.05).Conclusions: Cordycepin can markedly inhibit delayed type hypersensitivity with dosage-dependence after intraperitoneal injection at 24h interval (the last interval was 4 hours) possibly by its immunoregulative function.