| Objectives: To investigate the effects of LY294002, Phosphatidylinositol 3-kinase inhibition, on sperm motility in asthenozoospermia patients in vitro and further analyze the possible molecular mechanisms.Methods: Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 patients with idio-asthenozoospermia and 10 healthy fertile men were incubated with different concentrations of LY294002. Measurements of motility were carried out at 10min, 30min and 60 min in all specimens by CASA.Results: The sperm in idio-asthenozoospermia patients treated with LY294002 showed a significant increase in sperm progressive motility, the percentage of motile cells, VSL and VAP.Conclusions: LY294002 can enhance the motility of sperm in idio-asthenozoospermia patients in vitro.
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