Study On The Expression Of Mast Cells,Microvessels Density And Proliferating Cell Nuclear Antigen And Their Correlation In Cervical Carcinoma And Precancerous Lesions

【Objective】This study was to investigate the expression of mast cell (MC),endothelial marker of angiogenesis CD34 and proliferating cell nuclear antigen (PCNA) in normal cervical tissue, cervical intraepithelial neoplasm (CIN) and cervical carcinoma, and also investigated the relationship among mast cell density (MCD), microvessel density (MVD), proliferation index(PI)and clinical pathological factors, to analyze the correlation among them. The role of MC,CD34 and PCNA in the pathogenesis and development of cervical carcinoma and clinical significance was evaluated, and provide further theory evidence for clinical therapy of cervical carcinoma.【Methods】To collected 10 cases of normal cervical squamous epithelial tissue, 10 cases of CINⅠ, 10 cases of CINⅡ, 20 cases of CINⅢ(10 cases of cervical severe squamous dysplasia and carcinoma in suit respectively), 40 cases of invasive carcinoma of cervix(ICC)(these cases were classified according to Histology differentiation as follow: 24 cases of well and moderate differentiation, 16 cases of poor differentiation; Clinical stage was as follow: 22 cases of≤Ⅰb, 18 cases of ≥Ⅱa; The depth of invasion was as follow: 19 cases of <3㎝, 21 cases of≥3㎝, and there were 15 cases of ICC with lymph nodes metastasis. ). The expression of MC,CD34 and PCNA were detected by using S-P immunohistochemistry method in 90 cases of varies of cervical tissue, and count the amount of MCD,MVD and PI.【Results】(1) The MCD increased gradually in the order of normal tissue, CIN and cervical carcinoma in suit, and showed a significant difference among these groups(P<0.05), Compared with three groups of CIN , the difference had a extremely significant(P<0.01), although the MCD descended in ICC, it was higher than that in CINⅡ, and showed a significant difference between the two groups(P<0.05). The MCD did not show any statistical significance among CIN III, cervical carcinoma in suit and ICC(P>0.05). It was closely associated with the clinical stage of tumor, the depth of wall invasion and the metastasis of lymph nodes(P<0.05).(2) The MVD did not show any statistical significance between CIN I and normal tissue(P>0.05). The MVD increased remarkably in CINII and CINIII , and showed a significant differenc(eP<0.05). The MCD showed statistical significance among ICC, cervical carcinoma in suit and severe squamous dysplasia(P<0.05), and showed extremely significance among ICC, carcinoma in suit and severe squamous dysplasia(P<0.01). The MVD in the cases with poorly differentiated grade, highly clinical stage of tumor, deeply wall invasion and lymph node metastasis was significantly higher than that in the cases without the conditions mentioned above(P<0.05). (3) The PI in the normal tissue was the lowest, and in the ICC was the highest, in the order of groups, the PI was increased gradually, and showed a significant difference among these groups(P<0.05). The PI was closely associated with the clinical stage of tumor, the depth of wall invasion and the metastasis of lymph nodes (P<0.05).(4) Compared with the MVD in MCD high group and MCD low group, there was a extremely significance between them(P<0.01). The PI in MCD high group was obviously higher than that in MCD low group. A closely positive correlation was found between MCD and MVD(r=0.532,P<0.01), and the closely positive correlation was found between MCD and PI(r=0.480,P<0.0l), MVD and PI (r=0.619,P<0.0l)else.【Conclusion】The infiltrating MC around the cancer tissue may play a important role in the angiogenesis of tumor and the proliferation of tumor cell by releasing biological active substance. MCD,MVD and PCNA might be effective targets for the tumor progression, metastatic status and judging prognosis of the cervical carcinoma.