Screening Anti-tumor Medicine And Study On The Apoptotic Molecule Mechanism In HeLa Cell Induced By Betulinic Acid

Cell apoptosis is also named cell programmed death, which is one of the newest researches in biology. It is one of considerable vital phenomena in living nature. Apoptosis is closely associated with nemathelminth and high mammalia animal, embryo and adult, pathological and physiological conditions,life and die. It plays an important role in human health. As far as 100 years ago, Carl Vogt found this process. Kerr et al. have named it as"apoptosis"at 1972. Apoptosis is regulatoried by different genes, such as Ced gene family, bcl - 2 gene family, Fas/Apo gene family, P53 gene and ICE gene family etc.As one of Chinese traditional medicine, M arsdenia tenacissim a (Roxb) .Wight et Arn has been used for curing disease for thousands years in China. The factions and components were isolated from the fruits of M arsdenia tenacissim a (Roxb) .Wight et Arn. In our laboratory, those separate were screen for suppression activity of tumor cells. The result was shown that betulinic acid and betulin induced tumor cell died.This passage sieves medicine for curing tumor from constituents and chemical compounds are extracted from M arsdenia tenacissim a (Roxb) .Wight et Arn.This study on the apoptotic molecule mechanism in HeLa cell induced by betulinic acid. Do it with methods of crystal violet dying, RT-PCR were operated. Indicate the growth of tumor cells were significantly inhibited by betulin and betulinic acid in a time- and dose-dependent fashion. The furthers study indicated that betulinic acid induced HeLa cells apoptosis. The expression of P53 mRNA and P21 mRNA was increased and the expression of cyclinB1 mRNA was reduced in HeLa cells by Betulinic acid. But the expression of cyclinA mRNA was no change. The effect of Betulinic acid is mainly associated with apoptosis and cell cycle arrest.