Study Of Effects Of Adenosine In Ischemic Preconditioning In Rat Livers

ObjectiveTo investigate the relationship between the changes of adenosine concentration and ischemic tolerance resulted from ischemic preconditioning(IPC) in rat livers. So as to disclose the acquisition of ischemic tolerance of early preconditioning owing to the increase of adenosine.MethodsMale SD rats were used in our experiment. IPC group was administrated with 10-minute ischemia and then 10-minute reperfusion. During long-period ischemia and reperfusion, liver tissue and blood samples were collected at specific time. Concentration of adenosine, serum levels of liver-related enzymes were evaluated.ResultsAdenosine concentration increased dramatically in IPC group(P<0.05) during 45-minute ischema and subsequent reperfusion. Serum levels of liver-related enzymes descended in IPC group(P<0.05) during reperfusion.ConclusionsChanges of adenosine concentration is related to ischemic tolerance resulted from IPC. Increase of adenosine concentration was detected immediately after IPC and remained at a high level. This demonstrated the linkage between adenosine and early preconditioning.