The Relationship Between Leptin Receptor Polymorphism Gln223Arg, Leptin And Hypertensive Disorder Complicating Pregnancy

Objective: To investigate relationship between the clinical data of hypertensive disorder complicating pregnancy and levels of serum leptin include maternal and neonatal umbilical blood. And the relationship between leptin receptor polymorphism Gln223Arg, leptin and hypertensive disorder complicating pregnancy.Methods:1 Levels of serum leptin were measured in 32 hypertensive disorder complicating pregnant and 33 healthy pregnant women before the delivery time and their newborns by immunoradioassay methods.2 Immunohistochemical S-P method was employed to detect the expression of leptin in 33 cases of placenta from normal mature pregnancy and 50 cases of placenta from hypertention disorder complicating pregnancy.3 Using polymerase chain reaction-restriction fragment length polymorphism method to examine the Gln223Arg genotypes of LEPR among 33 cases of the normal pregnant women and 50 cases of those having got the hypertensive disorder complicating pregnancy.Results: 1 The levels of maternal and cord serum leptin about moderate and severe preeclampsia were higher than those in normal pregnant. And there was a significant difference between normal pregnant women and severe preeclampsia groups( P<0.05 ) . The positive correlations were significant between the leptin concentrations and fasting blood glucose of the both groups, especially in hypertensive disorder complicating pregnancy. In normal pregnancy, the leptin levels of cord serum blood were positively correlated with the newborn's body weight, body mass index.2 The serum leptin levels of GA and AA genotype of LEPR of the subjects having got hypertensive disorder complicating pregnancy is significantly higher than the subjects with GG genotype as compared.3 Under the microscopic examination, the placentas of hypertention disorder complicating pregnancy showed that vacuoles appeared in villous stroma, vasculo-syncytial membrane (VSM) was becoming thicker, the size of intervilli space was smaller, syncytial knots were hyperplastic, there were findings of fibrinoid necrosis of villi. The distribution of leptin in placenta of normal group is similar to that of hypertention disorder complicating pregnancy group. It was found in cytoplasm of villous syncytiotrophoblast and cellular of villous cytotrophoblast. And the expression of leptin in placentas was significantly higher of moderate and severe preeclampsia than normal group.Conclusion:1 The levels of maternal and cord serum leptin was high in hypertentive disorder complicating pregnancy. Serum leptin may play a role in the hypertentive disorder complicating pregnancy.2 The serum leptin levels of GA and AA genotype of LEPR of the subjects having got hypertensive disorder complicating pregnancy is significantly higher than the subjects with GG genotype as compared. And the A equivalent genotype is likely to be one of the indexes predicting the condition of hypertensive disorder complicating pregnancy.3 These results suggest that the abnormal expression of leptin may play a role in the hypertentive disorder complicating pregnancy. And it can reflect the function of placenta.