| Cervical cancer is one of the three most neoplasm of gynecology oncology, and the incidence rate is high in nation. The application of therapy is limited because of its unity and side effect. Thus, the study of nature products with low toxic in the treatment of cervical cancer is of cervical cancer is of great importance. As traditional Chinese medicines, the active components of Cyclocarya paliurus (Batal.) Iljinskjk, Ganoderma atrum, and Typhonieum giganteum Engler were said to be effective to some turmors, however, few studies were reported on their effects on the growth of cervical cancer. So this research investigated the regulate effects of natural products: polysaccharides isolated from Cyclocarya paliurus (Batal.) Iljinskjk (PCP), sterol from fruit body of Ganoderma atrum (SGA) and the solvent extracts of Typhonieum giganteum Engler. (STGE) on the growth of cervical cancer HeLa cells in vitro, and their anticancer mechanism. The results could provide theoretical foundation for further studies.Inverted microscope was used to observe the morphous of HeLa cells which were treated by PCP, SGA and STGE (STGE used in this experiment was obtained by different extraction methods) for 24 or 48 hours; MTT assay was applied to detect the proliferation activity of HeLa cells which were treated by PCP, SGA and STGE respectively; The apoptosis inducing effects of PCP, SGA and STGE to HeLa cells were tested using FCM analysis; flow cytometry was also used to detect the cell cycle of HeLa cells which treated by PCP and SGA; moreover, the expression of p16, p21, cyclinB1, bax, and bcl-2 in HeLa cells which stimulated with PCP and SGA were determined by RT-PCR. The results were as follows:①Cells stimulated with PCP grew sparsely and became round under the inverted microscope. Cell volume size was enlarged as time passing by. While SGA treated HeLa cells adhered lossly and many broken cells were existed. Cells stimulated with STGE obtained using different ways were in round shape, cell size was decreased and most of them were suspended in the culture medium, and the broken cells were increased with time, eventually, STGE A had more effects on Hela cells among the different STGE.②Concentration of PCP in 50, 100, 200, 400μg/mL and Concentration of SGA in 20, 40μg/mL could inhibit the growth of HeLa cells significantly (P＜0.1), and STGE obtained using different extraction methods in 100 mg/mL concentration (crude drug concentration) showed the same effects; the inhibition of SGA on HeLa cells was time and dose dependent.③FCM analysis indicated that the effects of PCP and SGA on inducing the apoptosis of HeLa cells were weak, but as the treating time of SGA increasing, the apoptosis ratio and die ratio of HeLa cells were increased slightly. The effects of STGE on inducing the apoptosis of HeLa cells were very strong, and cells in late apoptosis phase were increased as the treating time prolonged.④The results of flow cytometry analysis indicated that HeLa cells were significantly blocked in the S-phase after being treated for 24 hours by PCP, and the proportion of S-phase blocked cells increased as time prolonged; HeLa cells were blocked in the S-phase after being treated by SGA, but it was not obviously.⑤Test of the expression in HeLa cells which were respectively treated by PCP and SGA using RT-PCR method indicated that the total RNA extracted in each group were found expression ofβ-actin, p16, p21, cyclinB1, bax, and bcl-2; the expression ofβ-actin in each group was basically stable, and it could be used as a kind of internal parameter gene. The expression of p21, and bax in HeLa cells increased after stimulation of PCP and SGA respectively, while the expression of bcl-2 decreased.To concluded, PCP and SGA could significantly inhibit the growth of HeLa cells, blocked the cells in the S-phase and increase the expression of p21 and bax, while decrease the expression of bcl-2. Furthermore, STGE could inhibit the cell growth by inducing the apoptosis of HeLa cells significantly.
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